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09-241 Anti-phospho JAK-2 (Tyr570) Antibody

This Anti-phospho JAK-2 (Tyr570) Antibody is validated for use in WB, WB, WB for the detection of phospho JAK-2 (Tyr570).

Anti-phospho JAK-2 (Tyr570) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: JAK-2, JTK10 UniProt Number: O60674
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09-241
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Eq, Chp, Po, PmWB, WB, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-241
      DescriptionAnti-phospho JAK-2 (Tyr570) Antibody
      Alternate Names
      • Janus kinase 2
      • Janus kinase 2 (a protein tyrosine kinase)
      • tyrosine-protein kinase JAK2
      Background InformationJAK-2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK-2. JAK-2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK-2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Transfected HEK293T cell lysates
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho JAK-2 (Tyr570) Antibody is validated for use in WB, WB, WB for the detection of phospho JAK-2 (Tyr570).
      Key Applications
      • Western Blotting
      • Western Blotting
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JAK-2 phosphorylated at Tyr570.
      EpitopePhosphorylated Tyr570
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes JAK-2 when phosphorylated on Tyr570.
      Species Reactivity
      • Human
      • Rat
      • Horse
      • Chimpanzee
      • Pig
      • Primate
      Species Reactivity NoteDemonstrated to react with human and rat. Predicted to react with equine, chimpanzee, porcine, and primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq].
      Gene Symbol
      • JAK-2
      • JTK10
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION:Plays a role in leptin signaling and control of body weight By similarity. Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction.

      CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT STRUCTURE: Interacts with SIRPA and SH2B1 By similarity. Interacts with IL23R, SKB1 and STAM2.

      SUBCELLULAR LOCATION: Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.

      TISSUE SPECIFICITY: Expressed in blood, bone marrow and lymph node.

      DOMAIN: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.

      PTM:Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 by similarity.

      INVOLVEMENT IN DISEASE: Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome [MIM:600880]. Budd-Chiari syndrome is a spectrum of disease states, including anatomic abnormalities and hypercoagulable disorders, resulting in hepatic venous outflow occlusion. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain, and abdominal ascites.

      Defects in JAK2 are associated with polycythemia vera (PV) [MIM:263300]. PV, the most common form of primary polycythemia, is caused by somatic mutation in a single hematopoietic stem cell leading to clonal hematopoiesis. PV is a myeloproliferative disorder characterized predominantly by erythroid hyperplasia, but also by myeloid leukocytosis, thrombocytosis, and splenomegaly. Familial cases of PV are very rare and usually manifest in elderly patients.

      Defects in JAK2 gene may be a cause of essential thrombocythemia (ET) [MIM:187950]. ET is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.

      Defects in JAK2 are associated with familial myelofibrosis [MIM:254450]. Myelofibrosis with myeloid metaplasia is a myeloproliferative disease with annual incidence of 0.5-1.5 cases per 100,000 individuals and age at diagnosis around 60 (an increased prevalence is noted in Ashkenazi Jews). Clinical manifestations depend on the type of blood cell affected and may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension.

      Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.

      SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

      Contains 1 FERM domain.

      Contains 1 protein kinase domain.

      Contains 1 SH2 domain.
      Molecular Weight~ 125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in transfected HEK293T cell lysates.

      Western Blot Analysis: 0.05 ug/mL of this antibody detected JAK-2 on 10 µg of transfected HEK293T cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      09-241 04053252421518

      Documentation

      Anti-phospho JAK-2 (Tyr570) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho JAK-2 (Tyr570) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-JAK-2 (Tyr570) - 2444782 2444782
      Anti-phospho-JAK-2 (Tyr570) - 2032272 2032272
      Anti-phospho-JAK-2 (Tyr570) - 3211896 3211896
      Anti-phospho-JAK-2 (Tyr570) - 3502921 3502921
      Anti-phospho-JAK-2 (Tyr570) - 3639491 3639491
      Anti-phospho-JAK-2 (Tyr570) - 3821793 3821793
      Anti-phospho-JAK-2 (Tyr570) - 3914324 3914324
      Anti-phospho-JAK-2 (Tyr570) - 4116907 4116907
      Anti-phospho-JAK-2 (Tyr570) - DAM1757710 DAM1757710
      Anti-phospho-JAK-2 (Tyr570) -2772645 2772645
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      References

      Reference overviewPub Med ID
      Probiotics down-regulate genes in Salmonella enterica serovar typhimurium pathogenicity islands 1 and 2.
      Mohamed A Bayoumi,Mansel W Griffiths
      Journal of food protection  73  2010

      Show Abstract       		20202329 20202329

      Brochure

      Title
      Pathways and Biomarkers of JAK/STAT Signaling

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies