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382065 Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem

Cell-permeable, adenine-thymine-specific fluorescent stain.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342

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382065
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
23491-52-3C₂₇H₂₈N₆O · 3HCl

Pricing & Availability

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382065-100MG
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      Description
      OverviewCell-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei for fluorescence microscopy and flow cytometry applications. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H 33342 and the related compound H 33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10-fold greater cell-permeability than H 33258.
      Catalogue Number382065
      Brand Family Calbiochem®
      Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
      References
      ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
      Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
      Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
      Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
      Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
      Sandhu, L.C., et al. 1985. Cytometry 6, 191.
      Frankfurt, O.S. 1983. Cytometry 4, 216.
      Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
      Visser, J.W.M. 1980. Flow Cytometry IV, 86.
      Product Information
      CAS number23491-52-3
      ATP CompetitiveN
      FormYellow solid
      Hill FormulaC₂₇H₂₈N₆O · 3HCl
      Chemical formulaC₂₇H₂₈N₆O · 3HCl
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetAdenine-thymine-specific fluorescent stain
      Purity≥95% by TLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSDT4200000
      Safety Information
      R PhraseR: 23/24/25-36/37/38

      Toxic by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36/37/39-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Toxic & Carcinogenic / Teratogenic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      382065-100MG 04055977213089

      Documentation

      Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Bisbenzimide H 33342 Fluorochrome, Trihydrochloride - CAS 23491-52-3 - Calbiochem Certificates of Analysis

      TitleLot Number
      382065

      References

      Reference overview
      Werstuck, G., and Green, M.R. 1998. Science 282, 296.
      Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
      Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
      Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
      Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
      Sandhu, L.C., et al. 1985. Cytometry 6, 191.
      Frankfurt, O.S. 1983. Cytometry 4, 216.
      Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
      Visser, J.W.M. 1980. Flow Cytometry IV, 86.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-July-2008 RFH
      Synonyms2ʹ-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5ʹ-bi-1H-benzimidazole, 3HCl, HOE 33342
      DescriptionCell-permeable adenine-thymidine specific fluorescent stain. Useful for staining DNA, chromosomes or nuclei for fluorescence microscopy and flow cytometry applications. DNA-bound dye has an excitation maximum of 358 nm and an emission maximum of 460 nm. Useful for the simultaneous study of the cell cycle and apoptosis in unfixed cells. Induces cell differentiation. H33342 and the related compound H33258 (Cat. No. 382061) have been used in conjunction with RNA aptamers in the 5' untranslated region (UTR) to control expression of transfected gene constructs. Exhibits a 10 fold greater cell-permeability than H 33258.
      FormYellow solid
      CAS number23491-52-3
      RTECSDT4200000
      Chemical formulaC₂₇H₂₈N₆O · 3HCl
      Structure formulaStructure formula
      Purity≥95% by TLC
      SolubilityH₂O (50 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions (1 mg/ml) are stable for up to 2 weeks at 4°C. Precipitates in phosphate buffer solutions.
      Toxicity Toxic & Carcinogenic / Teratogenic
      ReferencesWerstuck, G., and Green, M.R. 1998. Science 282, 296.
      Paddenberg, R., et al. 1996. Eur. J. Cell Biol. 71, 105.
      Filatov, M.V., et al. 1995. Mutat. Res. 327, 209.
      Belloc, F., et al. 1994. Cytometry 17, 59. Crissman, H.A. 1990. Methods Cell Biol. 33, 97.
      Hinkley, R.E., et al. 1986. Dev. Biol. 118, 148.
      Sandhu, L.C., et al. 1985. Cytometry 6, 191.
      Frankfurt, O.S. 1983. Cytometry 4, 216.
      Lalande, M.E., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 363.
      Visser, J.W.M. 1980. Flow Cytometry IV, 86.

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      Alternative Products

      Catalog Number Description  
      382061 Bisbenzimide H 33258 Fluorochrome, Trihydrochloride - CAS 23491-45-4 - Calbiochem Show Pricing & Availability

      Categories

      Life Science Research > Inhibitors and Biochemicals > Biochemicals > Dyes, Stains and Probes > DNA-Binding Dyes