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QIA71 Caspase-8 Activity Assay Kit, Fluorometric

QIA71
  
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Overview

Key Specifications Table

Detection Methods
Fluorometric
Description
Overview

This product has been discontinued.





Suitable for the in vitro detection of caspase-8 activity in cell lysates. The assay is simple, rapid, and includes all controls. Suitable for use in frozen samples. Assay is validated against the Cell Death Detection NMP ELISA Kit (Cat. No. QIA20).
Catalogue NumberQIA71
Brand Family Calbiochem®
SynonymsICE-LAP5 Assay Kit, MACH Assay Kit, FLICE Assay Kit, CAP4 Assay Kit, CASP8 Assay Kit, Mch5 Assay Kit
Materials Required but Not Delivered 1 M dithiothreitol (DTT, Cleland reagent) solution (1.54 g to 10 ml H2O) made fresh (can be stored at -20°C for one month).
2-20 µl, 20-200 µl and 200-1000 µl precision pipettes with disposable tips.
Fluorescent plate reader capable of measuring excitation ~400 nm, and emission at ~505 nm.
A centrifuge, for spinning tubes or 96-well plate.
Microcentrifuge tubes.
References
ReferencesX.Sun, M., et al. 1999. J. Biol. Chem. 274, 5053.
Thornberry, N.A. and Lazebnik, Y. 1998. Science 281, 1312.
Martin, S.J. and Green, D.R. 1995. Cell 82, 349.
Product Information
Detection methodFluorometric
Form96 Tests
Format96-well plate
Kit contains96-Well Plate, Caspase-8 Extraction Buffer, Assay Buffer, Recombinant Caspase-8, Caspase-8 Substrate Conjugate, Caspase-8 Inhibitor, Control Cell Pellets, and a user protocol.
Biological Information
Assay time2-3 h
Sample TypeCell lysate
Safety Information
R PhraseR: 22-36/37/38-41

Harmful if swallowed.
Irritating to eyes, respiratory system and skin.
Risk of serious damage to eyes.
S PhraseS: 23-26-36

Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Intended useThe Calbiochem® Caspase-8 Activity Assay is a non-isotopic enzyme assay for the in vitro detection of Caspase-8 activity in cell lysates. The assay can be adapted for 96-well plates for sensitive drug screening methods.
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Storage ConditionsUpon Receipt of the kit: Store Recombinant Caspase-8 (2 vials) at -80°C to maintain stability. Store all other kit components at -20°C. Once thawed, Caspase-8 Sample Buffer and Assay Buffer may be stored at 4°C. Do not expose reagents to excessive light.
Protect from Moisture Protect from moisture
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Supplemental Information
Kit contains96-Well Plate, Caspase-8 Extraction Buffer, Assay Buffer, Recombinant Caspase-8, Caspase-8 Substrate Conjugate, Caspase-8 Inhibitor, Control Cell Pellets, and a user protocol.
Global Trade Item Number
Catalog Number GTIN
QIA71 0