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219498 Cathepsin S Substrate, Fluorogenic - Calbiochem

An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS, but not by Cathepsin B & L.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mca-GRWPPMG~LPWEK(Dnp)-D-R-NH2

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      Overview

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      Description
      Overview

      This product has been discontinued.



      An internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (kcat/Km = 1.018 µM-1 S-1), but not by Cathepsin B & L (kcat/Km = 0.004 and 0.032 µM-1 S-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages. (excitation maximum: 340 nm; emission maximum: 405 nm)
      Catalogue Number219498
      Brand Family Calbiochem®
      SynonymsMca-GRWPPMG~LPWEK(Dnp)-D-R-NH2
      References
      ReferencesLutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.
      Product Information
      FormYellow solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₉₃H₁₂₃N₂₅O₂₃S
      Chemical formulaC₉₃H₁₂₃N₂₅O₂₃S
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Peptide SequenceMca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      219498 0

      Documentation

      Cathepsin S Substrate, Fluorogenic - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Cathepsin S Substrate, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      219498

      References

      Reference overview
      Lutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-January-2011 RFH
      SynonymsMca-GRWPPMG~LPWEK(Dnp)-D-R-NH2
      DescriptionAn internally quenched, highly cathepsin S-selective, fluorogenic peptide substrate that is efficiently cleaved by CatS (kcat/Km = 1.018 µM-1 S-1), but not by Cathepsin B & L (kcat/Km = 0.004 and 0.032 µM-1 S-1, respectively). Studies using CatS-specific inhibitor LHVS reveal that the substrate is not cleaved by other proteolytic enzymes, including CatB, CatH, CatL, and CatX, present in endosomal extracts from human B cell line WT100, dendritic cells, and macrophages (excitation maximum: 340 nm; emission maximum: 405 nm).
      FormYellow solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₉₃H₁₂₃N₂₅O₂₃S
      Peptide SequenceMca-Gly-Arg-Trp-Pro-Pro-Met-Gly~Leu-Pro-Trp-Glu-Lys(Dnp)-D-Arg-NH₂
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesLutzner, N., and Kalbacher, H. 2008. J. Biol. Chem. 283, 36185.