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227039 Cholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B - Calbiochem

Cholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B, CAS 9012-63-9, is responsible for toxin binding to GM1 ganglioside receptors. When used alone, it blocks toxin action on intact cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Subunit of cholera toxin responsible for binding to GM1 ganglioside receptors in membranes. When used along, will block the action of toxin on intact cells.
      Catalogue Number227039
      Brand Family Calbiochem®
      References
      ReferencesMcCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol. 148, 137.
      Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
      Tsai, S.-C., et al. 1988. J. Biol. Chem. 263, 1768.
      Van Heynigen, S. 1982. Biosci. Rep. 2, 135.
      Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552.
      Gill, D.M. 1976. Biochemistry 15, 1242.
      Lai, C.Y., et al. 1976. J. Infect. Dis. 139, 674.
      Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294.
      Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.
      Product Information
      DeclarationNot available for sale outside of the United States.
      FormSolid
      FormulationLyophilized from 200 mM NaCl, 50 mM Tris-HCl, 3 mM NaN₃, 1 mM EDTA, pH 7.5.
      ReversibleN
      Quality LevelMQ100
      Applications
      ApplicationCholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B, CAS 9012-63-9, is responsible for toxin binding to GM1 ganglioside receptors. When used alone, it blocks toxin action on intact cells.
      Biological Information
      Biological activityCholera Toxin, B Subunit is tested for its effectiveness in binding antitoxin using the antitoxin combining power assay described by Craig, J.P., et al.
      Primary TargetGM1 ganglioside receptors
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 21/22-36/37/38

      Harmful in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 7-36/37

      Keep container tightly closed.
      Wear suitable protective clothing and gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Do not freeze Yes
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C. DO NOT FREEZE reconstituted solutions.
      End use certificateY
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      227039 0

      Documentation

      Cholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Cholera Toxin, B Subunit, Vibrio cholerae, Type Inaba 569B - Calbiochem Certificates of Analysis

      TitleLot Number
      227039

      References

      Reference overview
      McCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol. 148, 137.
      Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
      Tsai, S.-C., et al. 1988. J. Biol. Chem. 263, 1768.
      Van Heynigen, S. 1982. Biosci. Rep. 2, 135.
      Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552.
      Gill, D.M. 1976. Biochemistry 15, 1242.
      Lai, C.Y., et al. 1976. J. Infect. Dis. 139, 674.
      Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294.
      Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-September-2008 JSW
      DescriptionCholera toxin is an oligomeric protein consisting of a single A subunit (M.W. 28,000) containing two polypeptide chains, A1 and A2 , and a B subunit (M.W. 55,000) containing five polypeptide chains. Cholera toxin is the pathogenic agent responsible for the symptoms of cholera. Cholera toxin is also a potent activator of adenylate cyclase that binds the ADP-ribosylation factor and modulates its activity. The A subunit catalyzes the ADP-ribosylation of GS that leads to the persistent activation of adenylate cyclase. The consequent increase in intracellular cAMP levels stimulates Cl- secretion and leads to severe dehydration. The B subunit binds specifically to GM1 ganglioside receptors, which are expressed ubiquitously in mammalian cells, and assists in the transport of the A subunit through the membrane. When used alone, the B subunit will block the action of toxin on intact cells. Purified B subunit is produced by a modification of the methods published by Lai, C.Y., et al.
      FormSolid
      FormulationLyophilized from 200 mM NaCl, 50 mM Tris-HCl, 3 mM NaN₃, 1 mM EDTA, pH 7.5.
      Biological activityCholera Toxin, B Subunit is tested for its effectiveness in binding antitoxin using the antitoxin combining power assay described by Craig, J.P., et al.
      SolubilitySterile dH₂O.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 6 months at 4°C. DO NOT FREEZE reconstituted solutions.
      Toxicity Highly Toxic
      ReferencesMcCloskey, M.A. and Zhang, L. 2000. J. Cell. Biol. 148, 137.
      Mulhein, S.A., et al. 1989. J. Membr. Biol. 109, 21.
      Tsai, S.-C., et al. 1988. J. Biol. Chem. 263, 1768.
      Van Heynigen, S. 1982. Biosci. Rep. 2, 135.
      Mekalanos, J.J., et al. 1978. Infect. Immun. 20, 552.
      Gill, D.M. 1976. Biochemistry 15, 1242.
      Lai, C.Y., et al. 1976. J. Infect. Dis. 139, 674.
      Rappaport, R.S., et al. 1974. Infect. Immun. 9, 294.
      Craig, J.P. 1971. In Kadies, S., et al. (Eds.). Microbial Toxins. New York. Academic Press, 2A, 189.

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      Categories

      Life Science Research > Inhibitors and Biochemicals