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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
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-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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233155
MilliporeCleland’s Reagent - CAS 3483-12-3 - Calbiochem
A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.
More>>A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.
Oxidized dithiothreitol: ≤0.5%.
Catalogue Number
233155
Brand Family
Calbiochem®
Synonyms
Dithiothreitol, DTT
References
References
Gailis, L. 1994. Eur. J. Pharmacol. 292, 39. Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol.40, 125. Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199. Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716. Cleland, W.W. 1964. Biochemistry3, 480.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
29-April-2008 RFH
Synonyms
Dithiothreitol, DTT
Description
DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.
Form
White to off-white powder
Intert gas (Yes/No)
Packaged under inert gas
CAS number
3483-12-3
RTECS
EK1610000
Chemical formula
C₄H₁₀O₂S₂
Purity
≥97% by titration
Contaminants
Heavy metals: ≤1 ppm
Solubility
Ethanol or H₂O (30 mg/ml)
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Harmful
Merck USA index
14, 3376
References
Gailis, L. 1994. Eur. J. Pharmacol. 292, 39. Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol.40, 125. Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199. Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716. Cleland, W.W. 1964. Biochemistry3, 480.
