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234149 Collagen, Type I, Human Placenta

Collagen, Type I, Human Placenta MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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234149
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Overview

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234149-250UG
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      		 Plastic ampoule 250 μg
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      Description
      OverviewHighly purified, native, type I collagen from human placenta. Useful as a gel or thin coating for the attachment of cells. Recommended concentration: 0.2 - 2 µg/cm2 of growth surface.
      Catalogue Number234149
      Brand Family Calbiochem®
      References
      ReferencesBosco, M., et al. 1993. J. Cell Biol. 120, 537.
      Leavesley, D.I., et al. 1993. J. Cell Biol. 121, 163.
      Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys. 301, 103.
      Streuli, C.H., et al. 1993. J. Cell Biol. 120, 253.
      Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
      Klasson, S.C., et al. 1986. Coll. Relat. Res. 6, 397.
      Product Information
      CAS number9007-34-5
      FormLiquid
      FormulationIn 10 mM HCl.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      SourcePrepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      234149-250UG 04055977200027

      Documentation

      Collagen, Type I, Human Placenta SDS

      Title

      Safety Data Sheet (SDS) 

      Collagen, Type I, Human Placenta Certificates of Analysis

      TitleLot Number
      234149

      References

      Reference overview
      Bosco, M., et al. 1993. J. Cell Biol. 120, 537.
      Leavesley, D.I., et al. 1993. J. Cell Biol. 121, 163.
      Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys. 301, 103.
      Streuli, C.H., et al. 1993. J. Cell Biol. 120, 253.
      Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
      Klasson, S.C., et al. 1986. Coll. Relat. Res. 6, 397.

      Citations

      Title
    • Emily K. Thompson, et al. (2005) Endo180 binds to the C-terminal region of type I collagen. Journal of Biological Chemistry 280, 22596-22605.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-June-2010 JSW
      DescriptionHighly purified, native, type I collagen from human placenta. Useful as a gel or thin coating for the attachment of cells. Facilitates in vitro cultivation of cells and enhances cell-specific morphology and function. The recommended concentration is 0.2-2 µg/cm2 of growth surface.
      FormLiquid
      FormulationIn 10 mM HCl.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Coating Procedure Guidelines
      Note: The optimal concentration for cell attachment and culture may differ for different cell types, and experimentation may be required to determine the optimal conditions for your cell culture system.
      Human Collagen, Type I is typically used as a thin coating on plastic tissue culture plates. Alternatively, it may also be gelled.
      Guidelines for Coating Tissue Culture Plates (Thin Coating)
      1. Add sufficient volume of collagen to provide desired coating concentration. A coating concentration of 0.2 µg/cm2 to 2 µg/cm2 is recommended, depending on the cell type. It is important that the volume added to the dish is sufficient to cover the growth surface. If necessary, dilute the collagen stock with 2 mM HCl.
      2. Cover the growth surface completely covered and incubate for 2 h at room temperature.
      3. Tilt the dish just enough to allow excess collagen to drain to the lowest point in dish. Remove excess material with sterile pipette.
      4. Air dry the plate by leaving the lid slightly ajar in a laminar flow tissue culture hood or dry with a gentle stream of sterile gas.
      5. Once dry, the plates are ready for use.
      Guidelines for Gelling
      1. Dilute the collagen to desired concentration using 2 mM HCl.
      2. Mix 9 parts Collagen, Type I, Human Placenta and 1 part 10X buffer or 10X media.
      3. Add the mixture to the desired tissue culture vessel.
      4. Incubate for 15 to 60 min at 37°C. Gel is then ready for use, but must be handled carefully.
      SourcePrepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      CAS number9007-34-5
      Purity≥90% by SDS-PAGE
      SolubilityFurther dilute with acetic acid (10 mM)
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      Merck USA index14, 2480
      ReferencesBosco, M., et al. 1993. J. Cell Biol. 120, 537.
      Leavesley, D.I., et al. 1993. J. Cell Biol. 121, 163.
      Sidhu, J.S., et al. 1993. Arch. Biochem. Biophys. 301, 103.
      Streuli, C.H., et al. 1993. J. Cell Biol. 120, 253.
      Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
      Klasson, S.C., et al. 1986. Coll. Relat. Res. 6, 397.
      Citation
    • Emily K. Thompson, et al. (2005) Endo180 binds to the C-terminal region of type I collagen. Journal of Biological Chemistry 280, 22596-22605.
      		•