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341595 FGF-2, Basic, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli

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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥97% by SDS-PAGE
      Description
      OverviewRecombinant, human basic fibroblast growth factor expressed in E. coli. Potent mitogen for bone cells. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits inducible NOS in bovine retinal pigmented epithelial cells.
      Catalogue Number341595
      Brand Family Calbiochem®
      SynonymsrhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
      References
      ReferencesGoureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
      Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
      Li, J.J., et al. 1993. Cancer 72, 2253.
      Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
      Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
      Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
      Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
      Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
      Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
      Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
      Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
      Abraham, J.A., et al. 1986. EMBO J. 5, 2523.
      Product Information
      CAS number106096-93-9
      FormLyophilized
      FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg FGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSLK3641325
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      341595 0

      Documentation

      FGF-2, Basic, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      FGF-2, Basic, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      341595

      References

      Reference overview
      Goureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
      Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
      Li, J.J., et al. 1993. Cancer 72, 2253.
      Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
      Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
      Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
      Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
      Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
      Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
      Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
      Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
      Abraham, J.A., et al. 1986. EMBO J. 5, 2523.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-May-2008 RFH
      SynonymsrhbFGF, Fibroblast Growth Factor, Basic, Human, Recombinant, E. coli
      DescriptionRecombinant, human basic fibroblast growth factor expressed in E. coli. A member of the FGF family of mitogenic peptides, comprised of seven related proteins showing 35-55% amino acid conservation within the family and 55% similarity with acidic FGF. Found in neuronal tissue (hippocampus, brain stem, peripheral ganglia, and the isocortex), pituitary, adrenal cortex, corpus luteum, and placenta. Some of the biological activities attributed to basic FGF include: mitogenic activity in vitro for cells of meso- and neuroectodermal origin (i.e. fibroblasts, endothelial cells, astrocytes, oligodendrocytes, neuroblasts, keratinocytes, bovine epithelial lens cells, osteoblasts, smooth muscle cells, and melanocytes); role in wound healing; induction of neuron differentiation, survival, and regeneration; chemoattractant effect and mitogenic activity in vitro for endothelial cells and angiogenic activity in vivo; role in tumor neovascularization. Inhibits DNA synthesis and alkaline phosphatase activity in rat osteosarcoma cell lines. Has been implicated in the development of Kaposi sarcoma in vitro. Inhibits iNOS in bovine retinal pigmented epithelial cells. Binding of FGF to heparin or cell surface heparin sulfate proteoglycans is necessary for FGF binding to its high affinity receptors. Basic and acidic FGF appear to bind to the same high affinity receptors and show a similar range of biological activities.
      FormLyophilized
      FormulationLyophilized from sterile-filtered 20 mM Tris-HCl, 1 M NaCl, 50 µg BSA/1 µg cytokine, pH 7.0.
      CAS number106096-93-9
      RTECSLK3641325
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg FGF
      Biological activityED₅₀ = 100-250 pg/ml as monitored in a mitogenic assay by measuring FGF basic-dependent ³H-thymidine incorporation in quiescent NR6R/3T3 fibroblasts
      SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing 1 mM DTT and ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 6 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 4072
      ReferencesGoureau, O., et al. 1995. Eur. J. Biochem. 230, 1046.
      Gibran, N.S., et al. 1994. J. Surg. Res. 56, 226.
      Li, J.J., et al. 1993. Cancer 72, 2253.
      Mignatti, P., et al. 1992. J. Cell Physiol. 151, 81.
      Mignatti, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 11007.
      Baird, A., and Klagsbrun, M. 1991. Cancer Cells 3, 239.
      Gospodarowicz, D. 1991. Ann. NY Acad. Sci. 638, 1.
      Basilico, C., et al. 1989. Ann. NY Acad. Sci. 567, 95.
      Burgess, W.H., and Maciag, T. 1989. Annu. Rev. Biochem. 58, 575.
      Rogelj, S., et al. 1989. J. Cell. Biochem. 39, 13.
      Rizzino, A., et al. 1988. Cancer Res. 48, 4266.
      Abraham, J.A., et al. 1986. EMBO J. 5, 2523.

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      Categories

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors