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508940 G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: G418, G 418

508940
  
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #Empirical Formula
      108321-42-2C₂₀H₄₀N₄O₁₀ • 2H₂SO₄
      Description
      OverviewRecommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
      Catalogue Number508940
      Brand Family Calbiochem®
      SynonymsG418, G 418
      References
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Product Information
      CAS number108321-42-2
      FormLiquid
      FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.
      Hill FormulaC₂₀H₄₀N₄O₁₀ • 2H₂SO₄
      Chemical formulaC₂₀H₄₀N₄O₁₀ • 2H₂SO₄
      Quality LevelMQ200
      Applications
      Biological Information
      Purity≥98% by TLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 21/22

      Harmful in contact with skin and if swallowed.
      S PhraseS: 24-36

      Avoid contact with skin.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      508940 0

      Documentation

      G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      508940

      References

      Reference overview
      Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-December-2014 JSW
      SynonymsG418, G 418
      DescriptionRecommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
      FormLiquid
      FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.
      Intert gas (Yes/No) Packaged under inert gas
      CAS number108321-42-2
      Chemical formulaC₂₀H₄₀N₄O₁₀ • 2H₂SO₄
      Purity≥98% by TLC
      Storage Protect from light
      Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
      Toxicity Harmful
      ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
      Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
      Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
      Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
      Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
      Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
      Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
      Jimenez, A., and Davies, J. 1980. Nature 287, 869.