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F0483-BC HAT-Supplement

F0483-BC
  
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Overview

Description
OverviewHAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells. Hypoxanthine and thymidine supply preformed purines and pyrimidines for DNA synthesis by hybridomas via the salvage pathway that utilizes HGPRT- contributed by the fused spleen cell. Aminopterin, a folic acid antagonist, inhibits the de novo nucleoside biosynthesis pathway.

Specifications:

Sterility: Pass
pH: 9.0-11
Osmolality: 0.265-0.295 Osm/kg H20
Color: Colorless
Appearance: Clear

Catalogue NumberF0483-BC
Brand Family Calbiochem®
Product Information
Quality LevelMQ300
Storage and Shipping Information
Ship Code Dry Ice Only
Storage -20°C
Do not freeze Ok to freeze
Global Trade Item Number
Catalog Number GTIN
F0483-BC 0