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391338 HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.05.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid

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391338
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Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
7365-45-9C₈H₁₈N₂O₄S

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                      Description
                      OverviewA zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
                      Absorbance (1.0 M, H2O, 260 nm):≤0.05.
                      Absorbance (1.0 M, H2O, 280 nm): ≤0.04
                      Catalogue Number391338
                      Brand Family Calbiochem®
                      SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
                      References
                      Product Information
                      CAS number7365-45-9
                      FormWhite crystalline powder
                      Hill FormulaC₈H₁₈N₂O₄S
                      Chemical formulaC₈H₁₈N₂O₄S
                      PKA7.25 - 7.75 at 20°C
                      Structure formula ImageStructure formula Image
                      Quality LevelMQ100
                      Applications
                      Biological Information
                      Purity≥99% by titration (dry basis)
                      Physicochemical Information
                      ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
                      Heavy metals≤1 ppm
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      RTECSTL6809000
                      Safety Information
                      R PhraseR: 36/37/38

                      Irritating to eyes, respiratory system and skin.
                      S PhraseS: 26-37/39

                      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                      Wear suitable gloves and eye/face protection.
                      Product Usage Statements
                      Storage and Shipping Information
                      Ship Code Ambient Temperature Only
                      Toxicity Irritant
                      Storage +15°C to +30°C
                      Do not freeze Ok to freeze
                      Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
                      Packaging Information
                      Transport Information
                      Supplemental Information
                      Specifications
                      Global Trade Item Number
                      Catalog Number GTIN
                      391338-100GM 07790788049805
                      391338-1KG 07790788049812
                      391338-25GM 04055977190168
                      391338-500GM 07790788049829
                      391338-5KG 04055977212419

                      Documentation

                      HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem SDS

                      Title

                      Safety Data Sheet (SDS) 

                      HEPES, Free Acid, ULTROL® Grade - CAS 7365-45-9 - Calbiochem Certificates of Analysis

                      TitleLot Number
                      391338

                      Brochure

                      Title
                      Bulk packaging

                      Technical Info

                      Title
                      Molecular Biology Grade Reagents
                      Data Sheet

                      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                      Revision26-October-2009 RFH
                      SynonymsN-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid
                      DescriptionA zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
                      FormWhite crystalline powder
                      CAS number7365-45-9
                      RTECSTL6809000
                      Chemical formulaC₈H₁₈N₂O₄S
                      Structure formulaStructure formula
                      Purity≥99% by titration (dry basis)
                      ContaminantsChloride: ≤0.01%; Sodium: ≤0.1%; Sulfate: ≤0.05%
                      SolubilityH₂O (1 M)
                      Storage +15°C to +30°C
                      Do Not Freeze Ok to freeze
                      Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.
                      Toxicity Irritant
                      Merck USA index14, 4654

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