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407631 Interleukin-3, Mouse, Recombinant, E. coli

407631
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, mouse interleukin-3 expressed in E. coli. Stimulates production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils.

      Catalogue Number407631
      Brand Family Calbiochem®
      SynonymsrmIL-3
      References
      ReferencesMuther, H., et al. 1994. Growth Factors 10, 17.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered (0.22 µm) buffer, carrier-free.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 0.01-0.1 ng/ml as measured in a cell proliferation assay using NFS-60 cells.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤ 0.1 ng/µg IL-3
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      407631 0

      Documentation

      Interleukin-3, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Interleukin-3, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      407631

      References

      Reference overview
      Muther, H., et al. 1994. Growth Factors 10, 17.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-May-2008 RFH
      SynonymsrmIL-3
      DescriptionRecombinant, mouse interleukin-3 expressed in E. coli. IL-3 is a hemopoietic colony stimulating factor that interacts with very early multipotent hematopoietic progenitor cells. Stimulates production and differentiation of macrophages, neutrophils, basophils, mast cells, and eosinophils. IL-3 supports colony formation in the soft agar of bone marrow cells of granulocyte, macrophage, megakaryocyte, and erythrocyte lineages. Unlike human IL-3, the murine form supports the growth of mast cells. IL-3 activity is highly species-specific. Murine IL-3 does not show activity on human cells. Recombinant IL-3 produced in and purified from E. coli.
      FormLyophilized
      FormulationLyophilized from sterile-filtered (0.22 µm) buffer, carrier-free.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: ≤ 0.1 ng/µg IL-3
      Biological activityED₅₀ = 0.01-0.1 ng/ml as measured in a cell proliferation assay using NFS-60 cells.
      SolubilitydH₂O. Reconstitute to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low-endotoxin medium or buffer containing FBS or tissue culture grade BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      Merck USA index14, 4995
      ReferencesMuther, H., et al. 1994. Growth Factors 10, 17.