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681817 Lectin, Wheat Germ, Triticum vulgaris, Agarose Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: WGA-agarose

681817
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      WGA lectin immobilized on beaded agarose at 5 mg/ml. 1 ml WGA-agarose is pre-packed in a polypropylene column with 45 µm pore-size top and bottom frits. Each column also comes with a 1 ml negative control column packed with uncoupled agarose (activated agarose blocked with ethanolamine).
      Catalogue Number681817
      Brand Family Calbiochem®
      SynonymsWGA-agarose
      References
      ReferencesRousseau, C., et al. 1997. J. Cell Biochem. 66, 370.
      Natunen, J., et al. 1994. Glycobiology 4, 577.
      Valette, A., et al. 1987. Life Sci. 40, 535.
      Product Information
      FormulationPre-packed column in 150 mM NaCl, 10 mM sodium phosphate buffer, 0.05% NaN₃, pH 7.5.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      681817 0

      Documentation

      Lectin, Wheat Germ, Triticum vulgaris, Agarose Conjugate Certificates of Analysis

      TitleLot Number
      681817

      References

      Reference overview
      Rousseau, C., et al. 1997. J. Cell Biochem. 66, 370.
      Natunen, J., et al. 1994. Glycobiology 4, 577.
      Valette, A., et al. 1987. Life Sci. 40, 535.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsWGA-agarose
      DescriptionEach set contains one WGA lectin immobilized on agarose beads at 5 mg/ml. WGA-agarose is pre-packed in a polypropylene column with 45 µm pore-size top and bottom frits. Each column also comes with a 1 ml negative control column packed with uncoupled agarose (activated agarose blocked with ethanolamine). Each column contains 1 ml resin. Ready to use.
      FormulationPre-packed column in 150 mM NaCl, 10 mM sodium phosphate buffer, 0.05% NaN₃, pH 7.5.
      Recommended reaction conditionsMaterials Required: • Load/Equilibration Buffer: 20 mM sodium phosphate, pH 7.5, 150 mM NaCl • Wash Buffer: (same as Load/Equilibration buffer) • Elution Buffer: 20 mM sodium phosphate, pH 7.5, 150 mM NaCl, 100 mM N-Acetyl-α-D-glucosamine (GlcNAc, Cat. No. 1079) • Regeneration Buffer: (same as Load/Equilibration buffer) • Storage Buffer: 10 mM sodium phosphate, pH 7.5,150 mM NaCl, 0.05% NaN3 Protocol Note: Lectin column and negative control column should be used under identical conditions. 1. Buffers should be chilled to 4°C prior to use. Do not allow column to run dry. 2. Remove top and bottom caps from column. 3. Wash with 10 ml or more Equilibration Buffer. 4. Dissolve ligand in Equilibration Buffer to a concentration of ~2 mg/ml. Be sure that ligand is completely dissolved before proceeding. Clarify if necessary. 5. Apply ligand solution to column. 6. Wash with 20 ml or more Wash Buffer. Note: Some carbohydrates interact weakly with WGA. As a result, these carbohydrates are merely retarded by the immobilized WGA and will elute in the Wash Buffer. Wash Buffer fractions should be analyzed for the presence of carbohydrate. 7. Elute resin with a minimum of 5 ml Elution Buffer. 8. To reuse column, wash extensively (>20 ml) with Regeneration Buffer. 9. Wash column with 3 ml storage buffer. Store at 4°C.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRousseau, C., et al. 1997. J. Cell Biochem. 66, 370.
      Natunen, J., et al. 1994. Glycobiology 4, 577.
      Valette, A., et al. 1987. Life Sci. 40, 535.