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444224 MMP-2/MMP-9 Substrate II - Calbiochem

An exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B).

MMP-2/MMP-9 Substrate II - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ac-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II

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      Overview

      Replacement Information
      Description
      OverviewAn exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B). Also acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.
      Catalogue Number444224
      Brand Family Calbiochem®
      SynonymsAc-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
      References
      ReferencesWeingarten, H., et al. 1985. Anal. Biochem. 147, 437.
      Weingarten, H., et al. 1985. Biochemistry 24, 6730.
      Product Information
      ATP CompetitiveN
      FormWhite solid
      Hill FormulaC₃₁H₅₃N₅O₈S
      Chemical formulaC₃₁H₅₃N₅O₈S
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetSubstrate for the rapid, sensitive, and continuous spectrophotometric assay of Type IV collagenases, MMP-2 (gelatinase A), and MMP-9 (gelatinase B).
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableN
      Peptide SequenceAc-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      444224 0

      Documentation

      MMP-2/MMP-9 Substrate II - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      MMP-2/MMP-9 Substrate II - Calbiochem Certificates of Analysis

      TitleLot Number
      444224

      References

      Reference overview
      Weingarten, H., et al. 1985. Anal. Biochem. 147, 437.
      Weingarten, H., et al. 1985. Biochemistry 24, 6730.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2008 RFH
      SynonymsAc-Pro-Leu-Gly-(2-mercapto-4-methylpentanoyl)-Leu-Gly-OEt, Gelatinase A/Gelatinase B Substrate II
      DescriptionAn exceptional thiopeptide that is used as a substrate for the rapid, sensitive, and continuous spectrophotometric assay for Type IV collagenases MMP-2 (gelatinase A) and MMP-9 (gelatinase B). Acts as a substrate for vertebrate collagenase. Cleavage is monitored at 324 nm.
      FormWhite solid
      Recommended reaction conditions50 mM HEPES buffer, pH 6.5-7.0, 10 mM CaCl2, 0.5-1 mM 4,4'-dithiodipyridine, 0.01-5 mM substrate, and desired amount of activated enzyme; incubate at 25-26° while continuously monitoring cleavage at 324 nm.
      Chemical formulaC₃₁H₅₃N₅O₈S
      Peptide SequenceAc-Pro-Leu-Gly-SCH[CH₂CH(CH₃)₂]-CO-Leu-Gly-OC₂H₅
      Purity≥97% by HPLC
      Solubility50% acetic acid (10 mg/ml) or H₂O (1 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsUnstable in solution; reconstitute just prior to use.
      Toxicity Standard Handling
      ReferencesWeingarten, H., et al. 1985. Anal. Biochem. 147, 437.
      Weingarten, H., et al. 1985. Biochemistry 24, 6730.

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      Categories

      Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Protease Substrates