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PF118 MMP-2, Mouse Calvariae

MMP-2, Mouse Calvariae MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PF118
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Native MMP-2 from mouse calvariae. Pro-MMP-2 (progelatinase A) is cleaved in vivo by MT2-MMP to yield a 67 kDa intermediate and the 62 kDa active enzyme.
      Catalogue NumberPF118
      Brand Family Calbiochem®
      References
      ReferencesKnight, C.G., et al. 1992. FEBS Lett. 296, 215.
      Product Information
      Activity≥500 mU/mg after activation with APMA.
      Unit of DefinitionOne unit is defined as the amount of enzyme that can hydrolyze 1 µmol of the substrate N-(2,4)-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCI, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ®-35 Detergent, pH 7.0.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Substrate Cleavage Assay
      Zymography
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥400 mU/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PF118 0

      Documentation

      MMP-2, Mouse Calvariae SDS

      Title

      Safety Data Sheet (SDS) 

      MMP-2, Mouse Calvariae Certificates of Analysis

      TitleLot Number
      PF118

      References

      Reference overview
      Knight, C.G., et al. 1992. FEBS Lett. 296, 215.

      Brochure

      Title
      Art of Metastasis Poster PDF ( 610 KB )
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2007 RFH
      DescriptionNative MMP-2 from mouse calvariae. Pro-MMP-2 (progelatinase A) is cleaved in vivo by MT2-MMP to yield a 67 kDa intermediate and the 62 kDa active enzyme. Matrix metalloproteinase-2 (MMP-2) is involved in the degradation of several components of the extracellular matrix under various pathological conditions. In cancer tissues, MMP-2 is secreted by a proenzyme (proMMP-2), which is activated by membrane type 2 matrix metalloproteinase (MT2-MMP) on the cell surface. The 72 kDa pro-MMP-2 (progelatinase A) is cleaved by MT2-MMP to yield a 67 kDa intermediate and the 62 kDa active enzyme. Useful for immunoblotting, substrate cleavage assays, and zymography. Product may contain variable trace amount of active MMP-2.
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM Tris-HCI, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ®-35 Detergent, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions

      Organomercurial Activation Protocol This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature. The following protocol is from Stricklin, et al., which describes the use of p-aminophenylmercuric acetate (APMA) to activate pro-MMP. This protocol is also adaptable to other types of organomercurals, such as p-(hydroxymercuric) benzoate (PHMB), phenylmercuric chloride (PMC), or mersalyl. 1. Prepare a 10-50 mM stock solution of APMA (or other organomercurial compound) in 0.1 M NaOH just prior to use. Although not absolutely necessary, the stock solution may be adjusted to pH 11 with 5 N HCl (see Marcy, A.I., et al.). 2. To initiate the activation mix the proenzyme solution with the APMA solution at a 10:1 volume ratio (MMP:APMA). If a higher concentration of APMA is desired, increase the concentration of the stock solution. Do not exceed the 10:1 ratio, as this could result in significant changes in pH. 3. Incubate the mixture at 37°C for 2-3 h. It is recommended that an analytical run be conducted first to determine the optimal incubation time. For example, a small-scale experiment with a fixed concentration of pro-MMP and organomercurial would be incubated as described above. Remove aliquots of the sample at various time points during the incubation. Stop the reaction by the addition of SDS-PAGE sample buffer (e.g., 10 µl 2X sample buffer to 10 µl aliquot) and heat the samples to 95°C. The progress of activation can be monitored qualitatively by analyzing the aliquots on a 12% SDS-PAGE gel. 4. The activated MMP can be used without removing the APMA from the mixture. Please refer to Marcy, A.I., et al. for removal of organomercurials by gel filtration.
      Purity≥90% by SDS-PAGE
      Specific activity≥400 mU/mg protein
      Activity≥500 mU/mg after activation with APMA.
      Unit definitionOne unit is defined as the amount of enzyme that can hydrolyze 1 µmol of the substrate N-(2,4)-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
      Preservative≤0.1% sodium azide
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKnight, C.G., et al. 1992. FEBS Lett. 296, 215.