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PF117 MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MMP-24, Catalytic Domain

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PF117
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human pro-MT5-MMP fused to a His•Tag® sequence, expressed in E. coli and activated to yield the active catalytic domain. Two isoforms are produced, one with an N-terminal Tyr156, the other with an N-terminal Leu158. Useful for the study of the activation of progelatinase A and the degradation of extracellular matrix proteins. MT5-MMP is a member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. It has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
      Catalogue NumberPF117
      Brand Family Calbiochem®
      SynonymsMMP-24, Catalytic Domain
      References
      ReferencesLlano, E., et al. 1999. Cancer Res. 59, 2570.
      Pei, D. 1999. J. Biol. Chem. 274, 8925.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme necessary to hydrolyze 1.0 μmol of the substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 03-32-5032) per minute at 37°C, pH 7.5.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application NotesUseful for the investigation of the activation of progelatinase A and the degradation of extracellular matrix proteins.
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥100 mU/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PF117 0

      Documentation

      MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      MT5-MMP, Catalytic Domain, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      PF117

      References

      Reference overview
      Llano, E., et al. 1999. Cancer Res. 59, 2570.
      Pei, D. 1999. J. Biol. Chem. 274, 8925.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-July-2007 JSW
      SynonymsMMP-24, Catalytic Domain
      ApplicationUseful for the investigation of the activation of progelatinase A and the degradation of extracellular matrix proteins.
      DescriptionRecombinant, human pro-MT5-MMP fused to a His•Tag® sequence, expressed in E. coli and activated to yield the active catalytic domain. Two molecular species are produced, one with an N-terminal Tyr156 and the other with an N-terminal Leu158. M.W. (longer sequence): 23,064. Appears as a doublet of ~22,000 by SDS-PAGE. Useful for the investigation of the activation of progelatinase A and the degradation of extracellular matrix proteins.

      MT5-MMP (membrane-type matrix metalloproteinase-5, or MMP-24) is an ~74 kDa member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. MT5-MMP is expressed in adult brain, kidney, pancreas, and lung. Increased levels of MT5-MMP transcript have been found in astrocytomas, glioblastomas, and in pancreas and lung tumors. The enzyme has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
      BackgroundMT5-MMP (membrane-type matrix metalloproteinase-5, or MMP-24) is an ~74 kDa member of the Zn2+- and Ca2+-dependent endopeptidases that functions in the degradation of extracellular matrix components such as gelatin, chondroitin sulfate, and dermatan sulfate. MT5-MMP is expressed in adult brain, kidney, pancreas, and lung. Increased levels of MT5-MMP transcript have been found in astrocytomas, glioblastomas, and in pancreas and lung tumors. The enzyme has a tendency to be shed from cell surface membranes and appears to undergo rapid autocatalytic destruction into smaller fragments.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Specific activity≥100 mU/mg protein
      Unit definitionOne unit is defined as the amount of enzyme necessary to hydrolyze 1.0 μmol of the substrate MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 03-32-5032) per minute at 37°C, pH 7.5.
      PreservativeNone
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesLlano, E., et al. 1999. Cancer Res. 59, 2570.
      Pei, D. 1999. J. Biol. Chem. 274, 8925.