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AAMT004 Mitochondrial Complex IV (Human) Activity Assay Kit

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AAMT004
  
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      This product has been discontinued.



      Product Description

      The Mitochondrial Complex IV (Human) Activity Assay Kit is used to determine the activity of the cytochrome c oxidase enzyme (EC 1.9.3.1) in a human sample. Complex IV is immunocaptured within the wells and activity is determined colorimetrical by following the oxidation of reduced cytochrome c as an absorbance decrease at 550 nm. The overall reaction is as follows:



      4 cytochrome c- + 4 H+ + 4 H+(in) + O2 → 4 cytochrome c  + 2 H2O + 4 H+(out)

      reduced                    oxidized

      ↑Abs 550 nm               ↓Abs 550 nm


      Panel Components

      10 ml 	Buffer, (72571-10ML)
1 ml	Detergent, (72572-1ML)
1 ml	Reduced Cytochrome C, (72573-1ML)
1 ea	Microplate, (72574-1EA)

      *This kit contains sufficient matirials for 96 measurements (one 96-well microplate).

      Storage: Store the Buffer, Detergent, and Microplate at 4°C. Store the Reduced Cytochrome C at –20°C. For multiple experiments, Reduced Cytochrome C may be aliquoted and stored at –20°C or preferably at –80°C.

      This kit contains the necessary buffer, detergent for sample preparation, and substrate (reduced cytochrome c) for the reaction. The kit also contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.
      Catalogue NumberAAMT004
      Brand Family Novagen®
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      Ship Code Multiple Storage Conditions
      Storage Multiple storage requirements
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      Catalog Number GTIN
      AAMT004 0