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MAB324-K Mouse Anti-Neuron Specific Enolase, clone 5E2 (1mg)

Anti-Neuron Specific Enolase Antibody, clone 5E2 detects level of Neuron Specific Enolase & has been published & validated for use in ELISA, WB, IC, IH(P).

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MAB324-K
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      Description
      Catalogue NumberMAB324-K
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMouse Anti-Neuron Specific Enolase, clone 5E2 (1mg)
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-Neuron Specific Enolase
      References
      Product Information
      FormatPurified
      PresentationLiquid in PBS pH 7.4 containing 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-Neuron Specific Enolase Antibody, clone 5E2 detects level of Neuron Specific Enolase & has been published & validated for use in ELISA, WB, IC, IH(P).
      Key Applications
      • ELISA
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoblotting

      ELISA

      Immunohistochemistry. May be used on formalin-fixed, paraffin embedded sections.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenNeuron-specific enolase from human brain.
      Clone5E2
      Concentration1 mg/mL
      HostMouse
      SpecificityHuman gamma neuron specific enolase. Recognizes a 45kD band corresponding to gamma NSE on immunoblots of human brain extracts.
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
      Gene Symbol
      • ENO2
      • NSE
      • Gamma-enolase
      • EC 4.2.1.11
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09104 # Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
      COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer.
      SIZE: 434 amino acids; 47269 Da
      SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity).
      TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
      SIMILARITY: SwissProt: P09104 ## Belongs to the enolase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8 °C for up to 12 months under sterile conditions.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB324-K 04053252267123

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Other Primary Bulk Antibodies
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Neurological Proteins