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14-497 PKC η Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant amino acids 2-end, of human PKC eta. For use in Kinase Assays.

PKC η Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-497
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-497M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-497
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKC η Protein, active, 10 µg
      OverviewN-terminal His6-tagged, recombinant amino acids 2-end, of human PKC eta.

      PKC Lipid activator (Catalog # 20-133) is required for use.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant amino acids 2-end, of human PKC eta. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceHuman PKC eta expressed in Sf21 cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.
      Gene Symbol
      • PRKCH
      • PRKCL
      • PKCL
      • MGC26269
      • nPKC-eta
      • MGC5363
      • PKC-L
      Protein TargetPKCη
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24723 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
      SIZE: 682 amino acids; 77563 Da
      TISSUE SPECIFICITY: Most abundant in lung, less in heart and skin.
      DOMAIN: SwissProt: P24723 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
      DISEASE: SwissProt: P24723 # Defects in PRKCH may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight78.6kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of PKC Substrate Peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-497M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-497 04053252674617

      Documentation

      PKC η Protein, active, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      PKC η Protein, active, 10 µg Certificates of Analysis

      TitleLot Number
      PKC eta, active - 25609U-B 25609U-B

      FAQ

      QuestionAnswer
      Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.
      What type of buffer is best for lysis when attempting to preserve a protein?The best buffer is Buffer A. It is composed of 50mM Tris/HCl, pH 7.5, 1mM EGTA, 1mM EDTA, 1mM Sodium Orthovanadate (activated), 10mM Na B-glycerolphosphate, 50mM NaF, 5mM Na pyrophosphate, 1% w/v Triton X-100, 0.1% w/v 2-mercaptoethanol, 1ug/ml each of aprotinin, leupeptin and pepstatin, 1mM PMSF and 1mM microcystin (this is optional). QS with water. You may store Buffer A in aliquots at -20°C for 6 months.
      What is the Molar extinction coefficient for Adenosine 5'-[g32P]triphosphate ([g32P]ATP) used in the kinase assay?The Molar extinction coefficient is 15400.
      What is the best way to store and thaw enzymes?In order to maintain activity of enzymes, we recommend a slow thaw and aliquot storage. To do this, remove the full vial from the freezer and place under cold running tap water. Once thawed, place the vial on ice, gently mix, than make aliquots. Using liquid nitrogen or an ethanol/dry ice bath, flash freeze the aliquots. Store aliquots at the recommended temperature.
      When using p81 paper (phosphocellulose squares), what is the purpose of the acetone wash?The purpose of washing the p81 squares in acetone is mostly to help them dry faster, but it also can help to eliminate low specificity binding.
      Some of my enzyme preparations require Brij-35, what is its purpose?The role of Brij-35 is to function as a mild detergent, which prevents aggregation in active kinases.

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-497M PKC η Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases