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109272
Sigma-AldrichPapanicolaou's solution 3b polychromatic solution EA 50
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Description
Catalogue Number
109272
Description
Papanicolaou's solution 3b polychromatic solution EA 50
Overview
Papanicolaou’s solution 3b polychromatic solution EA 50 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65(Cat.no. 109270), o Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Papanicolaou’s solution 3b polychromatic solution EA 50 gives a blue-green-pinkish staining result in clinical specimen material. The 500 ml bottle provides 1500 - 2000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
H225: Highly flammable liquid and vapour. H302 + H312 + H332: Harmful if swallowed, in contact with skin or if inhaled. H319: Causes serious eye irritation. H370: Causes damage to organs.
Precautionary Statement(s)
P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water. P304 + P340 + P312: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor if you feel unwell. P308 + P311: IF exposed or concerned: Call a POISON CENTER/doctor. P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal Word
Danger
Storage class
3 Flammable liquids
WGK
WGK 2 obviously hazardous to water
Disposal
1 Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of danger
highly flammable, toxic
Product Usage Statements
Storage and Shipping Information
Storage
Store at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RID
UN 1987 , 3, II
Declaration (transport by air) IATA-DGR
UN 1987 , 3, II
Declaration (transport by sea) IMDG-Code
UN 1987 , 3, II
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)
passes test
Nuclei
blue to dark violet
Cyanophilic Cytoplasm (basophilic)
blue-green
Eosinophilic Cytoplasm (acidophilic)
pink
Global Trade Item Number
Catalog Number
GTIN
1092720500
04022536112886
1092721000
04022536676685
1092722500
04022536112909
Documentation
Papanicolaou's solution 3b polychromatic solution EA 50 SDS
