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109270
Sigma-AldrichPapanicolaou's solution 3c Polychromatic solution EA 65
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Description
Catalogue Number
109270
Description
Papanicolaou’s solution 3c polychromatic solution EA 65
Overview
Papanicolaou’s solution 3c polychromatic solution EA 65 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) and Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65(Cat.no. 109270), o Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. The Papanicolaou’s solution 3c polychromatic solution EA 65 will result in a staining with a light-brown to red staining result. The 100 ml bottle provides 300 - 400 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
H225: Highly flammable liquid and vapour. H317: May cause an allergic skin reaction. H319: Causes serious eye irritation.
Precautionary Statement(s)
P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233: Keep container tightly closed. P240: Ground and bond container and receiving equipment. P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water. P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal Word
Danger
Storage class
3 Flammable liquids
WGK
WGK 2 obviously hazardous to water
Disposal
1 Strongly contaminated halogen-free organic solvents: container A.
Safety Information
Categories of danger
highly flammable
Product Usage Statements
Storage and Shipping Information
Storage
Store at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RID
UN 1170 , 3, II
Declaration (transport by air) IATA-DGR
UN 1170 , 3, II
Declaration (transport by sea) IMDG-Code
UN 1170 , 3, II
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)
passes test
Nuclei
blue to dark violet
Cytoplasm
red
Global Trade Item Number
Catalog Number
GTIN
1092700100
04022536112824
Documentation
Papanicolaou's solution 3c Polychromatic solution EA 65 SDS
