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382156 PhosphoDetect™ Anti-Histone H1 Rabbit pAb

PhosphoDetect™ Anti-Histone H1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
382156
  
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      Overview

      Replacement Information

      Key Specifications Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~32 kDa phosphorylated histone H1 protein. Also detects proteins at ~64 kDa and ~130 kDa.
      Catalogue Number382156
      Brand Family Calbiochem®
      References
      ReferencesPennisi, E. 1997. Science 275, 155.
      Lu, M.J., et al. 1994. Chromosoma 103, 111.
      Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.
      Product Information
      FormLiquid
      Formulation105 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Not Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunoprecipitation (not recommended)
      Application CommentsIt has not yet been determined which specific histone H1 phosphorylation sites this antibody detects. Does not cross-react with unphosphorylated histone H1. Also detects bands at ~64 kDa and ~130 kDa that have been shown to contain histone H1 using anti-histone H1 blotting. For immunocytochemistry, colcemid-treated HeLa cells were fixed with 2% paraformaldehyde followed by cold methanol. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena hyperphosphorylated isoform of Tetrahymena histone H1
      ImmunogenTetrahymena
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      382156 0

      Documentation

      PhosphoDetect™ Anti-Histone H1 Rabbit pAb Certificates of Analysis

      TitleLot Number
      382156

      References

      Reference overview
      Pennisi, E. 1997. Science 275, 155.
      Lu, M.J., et al. 1994. Chromosoma 103, 111.
      Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-February-2008 JSW
      ApplicationImmunoblotting (1 µg/ml)
      Immunocytochemistry (5-10 µg/ml)
      Immunoprecipitation (not recommended)
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~32 kDa phosphorylated form of histone H1 protein.
      BackgroundHistone H1, the nonnucleosome linker histone, becomes phosphorylated at multiple sites (3-6 sites/molecule of H1) upon entry of the cell into mitosis, most likely by Cdc2 kinase. It has been proposed that H1 phosphorylation may allow for transient chromatin decondensations, thereby allowing access of specific DNA binding factors involved in gene activation, DNA replication and chromosome condensation to the DNA.
      HostRabbit
      Immunogen speciesTetrahymena
      Immunogena hyperphosphorylated isoform of Tetrahymena histone H1
      IsotypeIgG
      Speciesbovine, human
      FormLiquid
      Formulation105 mM NaCl, 70 mM Tris-glycine, 30% glycerol, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsIt has not yet been determined which specific histone H1 phosphorylation sites this antibody detects. Does not cross-react with unphosphorylated histone H1. Also detects bands at ~64 kDa and ~130 kDa that have been shown to contain histone H1 using anti-histone H1 blotting. For immunocytochemistry, colcemid-treated HeLa cells were fixed with 2% paraformaldehyde followed by cold methanol. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesPennisi, E. 1997. Science 275, 155.
      Lu, M.J., et al. 1994. Chromosoma 103, 111.
      Dedon, P.C., et al. 1991. Anal. Biol. Chem. 197, 83.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies