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524746 Phosphodiesterase 4D5, Active, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: cAMP-specific phosphodiesterase 4D isoform 5, PDE4D5

524746
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, human phosphodiesterdase 4D5 (PDE4D5) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE4D5 is expressed primarily in cardiac and vascular myocytes where it is found preferentially sequestered to β-arrestin. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
      Catalogue Number524746
      Brand Family Calbiochem®
      SynonymscAMP-specific phosphodiesterase 4D isoform 5, PDE4D5
      Application Data
      Increasing concentration of PDE inhibitor, rolipram, was added to enzyme and the activity assayed.
      References
      ReferencesBaillie., et al. 2007. Biochem J. 404, 71.
      Lynch, M.J., et al. 2005. J. Biol. Chem. 280,33178.
      Bolger, G.B., et al. 2002. BMC Biochem. 3, 24.
      Product Information
      Activity≥ 0.5 U/µl
      Unit of DefinitionOne unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5 at 30°C.
      FormLiquid
      Formulation150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      524746 0

      Documentation

      Phosphodiesterase 4D5, Active, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      524746

      References

      Reference overview
      Baillie., et al. 2007. Biochem J. 404, 71.
      Lynch, M.J., et al. 2005. J. Biol. Chem. 280,33178.
      Bolger, G.B., et al. 2002. BMC Biochem. 3, 24.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-March-2009 JSW
      SynonymscAMP-specific phosphodiesterase 4D isoform 5, PDE4D5
      Application Data
      Increasing concentration of PDE inhibitor, rolipram, was added to enzyme and the activity assayed.
      DescriptionFull-length, recombinant, human phosphodiesterdase 4D5 (PDE4D5) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE4D5 is expressed primarily in cardiac and vascular myocytes where it is found preferentially sequestered to β-arrestin. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
      FormLiquid
      Formulation150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3
      Activity≥ 0.5 U/µl
      Unit definitionOne unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5 at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBaillie., et al. 2007. Biochem J. 404, 71.
      Lynch, M.J., et al. 2005. J. Biol. Chem. 280,33178.
      Bolger, G.B., et al. 2002. BMC Biochem. 3, 24.