Qty	Catalog Number	Ordering Description	Qty/Pack	List Price
			96-Well Plate

Qty	Catalog Number	Ordering Description	Qty/Pack	List Price
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

537059 Sigma-AldrichPropidium Iodide - CAS 25535-16-4 - Calbiochem

Membrane impermeable DNA intercalator.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet
Citations

Recommended Products

Overview

Replacement Information

Key Specifications Table

CAS #	Empirical Formula
25535-16-4	C₂₇H₃₄N₄I₂

Pricing & Availability

Catalog Number	Availability	Packaging	Qty/Pack	Price	Quantity
537059-100MG	Retrieving availability... — Limited Availability Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Limited Availability Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Glass bottle	100 mg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
537059-1GM	Retrieving availability... — Limited Availability Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Limited Availability Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	1 gm	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
537059-250MG	Retrieving availability... — Limited Availability Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Limited Availability Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Plastic ampoule	250 mg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites
537059-50MG	Retrieving availability... — Limited Availability Stocked Discontinued Limited Quantities Available Available Login to See Inventory Stocked Limited Availability Stocked Remaining : Will advise Remaining : Will advise Will advise Contact Customer Service Contact Customer Service Contact Customer Service Contact Customer Service	Glass bottle	50 mg	Retrieving price... Price could not be retrieved Minimum Quantity is a multiple of Maximum Quantity is Upon Order Completion More Information You Saved () — Request Pricing Login to See Your Pricing	Check Availability —	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Overview	Extinction coefficient(493 nm): 5900 M ^-1cm^-1;(287 nm): 55,700 M ^-1cm^-1;
Catalogue Number	537059
Brand Family	Calbiochem®

References
References	Belloc, F., et al. 1994. Cytometry 17, 59. Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11. Pollice, A.A., et al. 1992. Cytometry 13, 432.

Product Information
CAS number	25535-16-4
ATP Competitive	N
Form	Dark red solid
Hill Formula	C₂₇H₃₄N₄I₂
Chemical formula	C₂₇H₃₄N₄I₂
Reversible	N
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Primary Target	Membrane impermeable DNA intercalator
Sample Type	Intact Cells

Physicochemical Information
Cell permeable	N
Emission max.
Excitation max.

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	SF7949600

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Irritant
Storage	+2°C to +8°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
537059-100MG	04055977195897
537059-1GM	04055977195880
537059-250MG	04055977195903
537059-50MG	04055977195910

Documentation

Propidium Iodide - CAS 25535-16-4 - Calbiochem SDS

Title
Safety Data Sheet (SDS)

Propidium Iodide - CAS 25535-16-4 - Calbiochem Certificates of Analysis

Title	Lot Number
537059	Enter Lot Number

References

Reference overview
Belloc, F., et al. 1994. Cytometry 17, 59. Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11. Pollice, A.A., et al. 1992. Cytometry 13, 432.

Citations

Title

Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.

Tirado, O.M., et al. 2005. Cancer Research 65, 9320.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	29-August-2008 RFH
Description	Membrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M^-1cm^-1 (493 nm); 55,700 M^-1cm^-1 (287 nm); 13,800 M^-1cm^-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
Form	Dark red solid
CAS number	25535-16-4
RTECS	SF7949600
Chemical formula	C₂₇H₃₄N₄I₂
Structure formula
Solubility	H₂O. Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
Storage	Protect from light +2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity	Irritant
References	Belloc, F., et al. 1994. Cytometry 17, 59. Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11. Pollice, A.A., et al. 1992. Cytometry 13, 432.
Citation	Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953. Tirado, O.M., et al. 2005. Cancer Research 65, 9320.

The Product Has Been Added To Your Cart