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539785 ProteoExtract® Partial Yeast Proteome Extraction Kit

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: P-PEK Yeast Kit

539785
  
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      Overview

      Replacement Information
      Description
      OverviewThe Partial Yeast Proteome Extraction Kit (P-PEK, Yeast) is designed to extract proteins of variable solubility from yeast cells in the presence of different detergents and chaotropes. Each of the provided extraction reagents solubilizes a different subset of cellular proteins. The most soluble of the yeast proteins are released by mechanical destruction of the cells with Extraction Reagent 1, then the membrane proteins can be extracted using Extraction Reagent 3. These fractions can be analyzed on separate 2-D gel electrophoresis, and the insoluble fraction can be analyzed by one-dimensional SDS-PAGE using the SDS buffers provided. To preserve the protein profile and increase spot resolution, the kit also contains Protease Inhibitor Cocktail and the proprietary non-specific Benzonase® Nuclease (Cat. No. 71206).
      Note: 1 kit is sufficient for up to 20 sample extractions.
      Catalogue Number539785
      Brand Family Calbiochem®
      SynonymsP-PEK Yeast Kit
      References
      ReferencesYuan, X., et al. 2002. Electrophoresis 23, 1185.
      Product Information
      Form20 Extractions
      Kit containsWash buffer, Extraction Reagent 1, Rehydration Reagent 2, Extraction Reagent 3A, Extraction Reagent 3B, SDS Buffer A, SDS Buffer B, glass beads, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
      Applications
      Biological Information
      Sample TypeYeast cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage FREEZER (-20°C)
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsWash buffer, Extraction Reagent 1, Rehydration Reagent 2, Extraction Reagent 3A, Extraction Reagent 3B, SDS Buffer A, SDS Buffer B, glass beads, Protease Inhibitor Cocktail, Benzonase® Nuclease (Cat. No. 71206), and a user protocol.
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      539785 0

      Documentation

      References

      Reference overview
      Yuan, X., et al. 2002. Electrophoresis 23, 1185.

      User Protocols

      Title
      User Protocol