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556785 RNase T₁, Aspergillus oryzae

RNase T₁, Aspergillus oryzae MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ribonuclease T₁

556785
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



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      Native RNase T1 from Aspergillus oryzae. Guanylate-specific ribonuclease that hydrolyzes the 3′-phosphate of guanylate residues in polynucleotides. Guanosine-3′-phosphates and oligonucleotides with guanosine-3′-phosphate terminal groups are the final products. Note: 1 TU = 1000 units.
      Catalogue Number556785
      Brand Family Calbiochem®
      SynonymsRibonuclease T₁
      References
      ReferencesWaltz, F.G., Jr. 1997. Biochim. Biophys. Acta 1350, 183.
      Plaza del Pino, I.M., et al. 1992. Biochemistry 31, 11196.
      Uchimavu, T., et al. 1992. Biochem. Biophys. Res. Commun. 187, 1523.
      Zegers, I., et al. 1992. Biochemistry 31, 11317.
      Egami, F., et al. 1964. Prog. Nucleic Acid Res. Mol. Biol. 3, 59.
      Product Information
      CAS number9026-12-4
      Activity≥200 units/mg dry weight
      Unit of DefinitionAccording to Egami et al. (1964) one unit will increase the absorbance of the acid-soluble fraction of the 0.3% yeast RNA solution by A₂₆₀ = 1 in 15 min at 37°C, pH 7.5 in a reaction mixture of 1 ml. One Calbiochem unit equals 30 Egami units.
      EC number3.1.27.3
      FormLyophilized
      Applications
      Biological Information
      Physicochemical Information
      ContaminantsDNase, mono- and diesterase: ≤0.02%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSRF0753000
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      556785 0

      Documentation

      RNase T₁, Aspergillus oryzae Certificates of Analysis

      TitleLot Number
      556785

      References

      Reference overview
      Waltz, F.G., Jr. 1997. Biochim. Biophys. Acta 1350, 183.
      Plaza del Pino, I.M., et al. 1992. Biochemistry 31, 11196.
      Uchimavu, T., et al. 1992. Biochem. Biophys. Res. Commun. 187, 1523.
      Zegers, I., et al. 1992. Biochemistry 31, 11317.
      Egami, F., et al. 1964. Prog. Nucleic Acid Res. Mol. Biol. 3, 59.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-June-2008 JSW
      SynonymsRibonuclease T₁
      DescriptionNative RNase T1 from Aspergillus oryzae. Ribonuclease that cleaves the phosphodiester bond between the 3'-phosphate of guanylate residues and the 5'-hydroxy group of the adjacent nucleotide. The reaction, which involves the formation of 2'3'-cyclic guanylate intermediate, splits RNA into 3'-GMP and oligonucleo-tides with a terminal 3'-GMP.

      Note: 1 TU = 1000 units.
      FormLyophilized
      CAS number9026-12-4
      RTECSRF0753000
      EC number3.1.27.3
      ContaminantsDNase, mono- and diesterase: ≤0.02%
      Activity≥200 units/mg dry weight
      Unit definitionAccording to Egami et al. (1964) one unit will increase the absorbance of the acid-soluble fraction of the 0.3% yeast RNA solution by A₂₆₀ = 1 in 15 min at 37°C, pH 7.5 in a reaction mixture of 1 ml. One Calbiochem unit equals 30 Egami units.
      SolubilityH₂O and Aqueous Buffers
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 8202
      ReferencesWaltz, F.G., Jr. 1997. Biochim. Biophys. Acta 1350, 183.
      Plaza del Pino, I.M., et al. 1992. Biochemistry 31, 11196.
      Uchimavu, T., et al. 1992. Biochem. Biophys. Res. Commun. 187, 1523.
      Zegers, I., et al. 1992. Biochemistry 31, 11317.
      Egami, F., et al. 1964. Prog. Nucleic Acid Res. Mol. Biol. 3, 59.