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HTS123RTA Ready-to-Assay™ BB1 Bombesin Receptor Frozen Cells

Human BB1 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_002511
HTS123RTA
1 kit  
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      Overview

      Replacement Information

      Key Specifications Table

      SpeciesHost CellsProtein Target
      Human Chem-1BB1
      Description
      Catalogue NumberHTS123RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ BB1 Bombesin Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB). A family of 3 GPCRs, including NMB-R (BB1), GRP-R (BB2) and BRS-3 (BB3), mediate the biological effects of the peptides. The receptors differ in their affinities for the peptides; BB2 binds to GRP with 50-300-fold greater affinity than to NMB, whereas BB1 binds to NMB with 10-800-fold greater affinity than to GRP (Tokita et al., 2004). Binding of ligand to BB1 activates Gq to increase intracellular calcium concentrations. The CNS is a major site of NMB and BB1 expression, and BB1 appears to be involved in thermoregulation (Ohki-Hamazaki et al., 2005).Millipore’s cloned human BB1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant BB1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at BB1.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ100
      Applications
      ApplicationHuman BB1 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetBB1
      Target Sub-FamilyBombesin
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      HTS123RTA 04053252552496

      Documentation

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells