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69060 S-Protein FITC Conjugate

69060
  
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      This product has been discontinued.





      The S-protein FITC Conjugate is designed for in situ fluorescent detection of S•Tag™ fusion proteins. It has been optimized for maximum signal without loss in S-protein binding activity. Because of its small size, this conjugate penetrates cells rapidly. This makes it ideal for staining insect or mammalian cells to confirm recombinant protein expression. The 200 µl size will stain a minimum of 200 slides. The recommended working dilution for cell staining is 1:2,000.
      Catalogue Number69060
      Brand Family Novagen®
      Application Data
      S-protein FITC detection of baculovirus-expressed S·Tag fusion protein in infected Sf9 cells A BacVector® recombinant baculovirus expressing an S·Tag fusion protein was used to infect Ready-Plaque Sf9 Cells. Forty-eight hours after infection, the cells were processed and stained with S-protein FITC. Left panel, fluorescent illumination; right panel, phase contrast photo of the same field. Lower right insets are uninfected control cells. The Western blot insert in the right panel was performed with a total cell extract loaded in parallel with Novagen's Perfect Protein™ Markers and developed with S-protein AP Conjugate, which recognizes both the S·Tag fusion protein product and the markers.
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      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
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      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      69060 0

      Documentation

      S-Protein FITC Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      S-Protein FITC Conjugate Certificates of Analysis

      TitleLot Number
      69060

      Brochure

      Title
      Protein Purification and Detection Tools

      Citations

      Title
    • Malla Padidam, Roger N. Beachy and Claude E. Fauquet. (1999) A phage single-stranded DNA (ssDNA) binding protein complements ssDNA accumulation of a geminivirus and interferes with viral movement. Journal of Virology 73, 1609-1616.
    • N.C. Chi and S.A. Adam. (1997) Functional domains in nuclear import factor p97 for binding the nuclear localization sequence receptor and the nuclear pore. Molecular Biology of the Cell 8, 945-956.
    • User Protocols

      Title
      TB143

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
      Life Science Research > Protein Detection and Quantification > Western Blotting > Western Blotting Kits and Reagents