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69999 T7•Tag® Antibody AP Conjugate

69999
  
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      Overview

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      Description
      Overview

      This product has been discontinued.



      The T7•Tag AP Conjugate is useful as a single detection reagent and eliminates the need for second antibodies or streptavidin conjugates. The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

      The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

      This antibody is also available labeled with biotin and as a direct conjugate with horseradish peroxidase.

      SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
      Species/IsotypeMouse monoclonal IgG2b
      Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
      Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
      < 1 ng (developed with chemiluminescent substrates)
      FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
      Working dilution1:5,000–1:10,000

      Catalogue Number69999
      Brand Family Novagen®
      References
      Product Information
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      Biological Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      69999 0

      Documentation

      T7•Tag® Antibody AP Conjugate Certificates of Analysis

      TitleLot Number
      69999

      Brochure

      Title
      Protein Purification and Detection Tools

      Citations

      Title
    • Nadia L. Korneeva, et al. (2005) Interaction between the NH2-terminal domain of eIF4A and the central domain of eIF4G modulates RNA-stimulated ATPase activity. Journal of Biological Chemistry (280) 1872-1881.
    • S. Dean Rider, et al. (2005) The protozoan parasite Cryptosporidium parvum possesses two functionally and evolutionarily divergent replication protein A large subunits. Journal of Biological Chemistry 280, 31460-31469.
    • User Protocols

      Title
      TB112 T7•Tag® Antibody AP Conjugate

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies