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616379 Toxin A, Clostridium difficile - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Enterotoxin

616379
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
      Catalogue Number616379
      Brand Family Calbiochem®
      SynonymsEnterotoxin
      References
      ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
      Mahida, Y.R., et al. 1996. Gut 38, 337.
      Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
      Product Information
      ATP CompetitiveN
      FormSolid
      FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityBinds to fresh rabbit red blood cells in a hemagglutination assay. Cytotoxic as determined by a cell rounding assay using human foreskin cells
      Primary TargetSmall GTPases, such as Rho, Rac, and Cdc42
      PuritySingle band by SDS-PAGE
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSKA8080200
      Safety Information
      R PhraseR: 26/27/28-36/37/38

      Very toxic by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36/37/39-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Highly Toxic
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C. Toxin A activity was found to decrease to 1/10 after one week storage at 4°C following reconstitution.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      616379 0

      Documentation

      Toxin A, Clostridium difficile - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Toxin A, Clostridium difficile - Calbiochem Certificates of Analysis

      TitleLot Number
      616379

      References

      Reference overview
      Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
      Mahida, Y.R., et al. 1996. Gut 38, 337.
      Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-May-2009 RFH
      SynonymsEnterotoxin
      DescriptionA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
      FormSolid
      FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
      RTECSKA8080200
      PuritySingle band by SDS-PAGE
      Biological activityBinds to fresh rabbit red blood cells in a hemagglutination assay. Cytotoxic as determined by a cell rounding assay using human foreskin cells
      SolubilityReconstitute with 50 µl of sterile, distilled H₂O.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 1 week at 4°C. Toxin A activity was found to decrease to 1/10 after one week storage at 4°C following reconstitution.
      Toxicity Highly Toxic
      ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
      Mahida, Y.R., et al. 1996. Gut 38, 337.
      Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.