Millipore Sigma Vibrant Logo

662098 UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: UBD2D3, Ubiquitin-conjugating enzyme E2 D3

View Products on Sigmaaldrich.com
662098
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      OverviewFull-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      Catalogue Number662098
      Brand Family Calbiochem®
      SynonymsUBD2D3, Ubiquitin-conjugating enzyme E2 D3
      References
      ReferencesBrzovic, P.S., et al. 2006. Cell Cycle 5, 2867.
      Gehrke, S.G., et al. 2003 Blood. 101, 3288.
      Nuber, U., And Scheffner, M., 1999. J. Biol. Chem. 274, 7576.
      Schwarz, S.E., et al. 1998. J. Biol. Chem. 273, 12148.
      Jensen, J., et al. 1995. J. Biol. Chem. 270, 30408.
      Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci. 91, 8797.
      Product Information
      FormulationIn 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      662098 0

      Documentation

      UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      662098

      References

      Reference overview
      Brzovic, P.S., et al. 2006. Cell Cycle 5, 2867.
      Gehrke, S.G., et al. 2003 Blood. 101, 3288.
      Nuber, U., And Scheffner, M., 1999. J. Biol. Chem. 274, 7576.
      Schwarz, S.E., et al. 1998. J. Biol. Chem. 273, 12148.
      Jensen, J., et al. 1995. J. Biol. Chem. 270, 30408.
      Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci. 91, 8797.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-May-2009 JSW
      SynonymsUBD2D3, Ubiquitin-conjugating enzyme E2 D3
      DescriptionFull-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      FormulationIn 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsTypical concentration for conjugate formation: 100 nM to 1 µM depending on the conditions
      Purity≥95% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesBrzovic, P.S., et al. 2006. Cell Cycle 5, 2867.
      Gehrke, S.G., et al. 2003 Blood. 101, 3288.
      Nuber, U., And Scheffner, M., 1999. J. Biol. Chem. 274, 7576.
      Schwarz, S.E., et al. 1998. J. Biol. Chem. 273, 12148.
      Jensen, J., et al. 1995. J. Biol. Chem. 270, 30408.
      Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci. 91, 8797.