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559324 p38α, His•Tag®, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: p38α, CSBP, Mxi2, p38, RK, SAPK2α

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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Recombinant, human p38α/SAPK2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified SAP Kinase in vitro with MKK6. Suitable for labeling SAP Kinase substrates.
      Catalogue Number559324
      Brand Family Calbiochem®
      Synonymsp38α, CSBP, Mxi2, p38, RK, SAPK2α
      References
      ReferencesGuo, X., et al. 2003. J. Biol. Chem. 278, 22237.
      Sanz-Moreno V., et al. 2003. Mol. Cell Biol. 23, 3079.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol of phosphate to myelin basic protein (MBP) per min at 30°C.
      FormLiquid
      FormulationIn 150 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥12,000 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsProteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      559324 0

      Documentation

      p38α, His•Tag®, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      p38α, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      559324

      References

      Reference overview
      Guo, X., et al. 2003. J. Biol. Chem. 278, 22237.
      Sanz-Moreno V., et al. 2003. Mol. Cell Biol. 23, 3079.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-September-2008 RFH
      Synonymsp38α, CSBP, Mxi2, p38, RK, SAPK2α
      DescriptionRecombinant, human p38α/SAPK2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified SAPK2α in vitro with MKK6. Suitable for labeling SAPK2α substrates.
      FormLiquid
      FormulationIn 150 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions20 mM Tris-HCl or MOPS, 18.75 mM MgCl2, 25 mM β-glycerolphosphate, 5 mM EGTA, 1 mM sodium orthovanadate, 1 mM DTT, 0.5 mg/ml myelin basic protein, 125 µM ATP (0.0375 µCi/µl), pH 7.5, 30°C.
      Purity≥95% by SDS-PAGE
      ContaminantsProteases: none detected
      Specific activity≥12,000 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol of phosphate to myelin basic protein (MBP) per min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesGuo, X., et al. 2003. J. Biol. Chem. 278, 22237.
      Sanz-Moreno V., et al. 2003. Mol. Cell Biol. 23, 3079.