Millipore Sigma Vibrant Logo

70791 pET Dsb Fusion System 40b

Vectors for export and periplasmic folding of target proteins

pET Dsb Fusion System 40b MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70791
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The pET Dsb Fusion Systems 39b and 40b are designed for cloning and high-level expression of peptide sequences fused with the 208-aa DsbA•Tag™ sequence [pET-39b(+)] or the 236-aa DsbC•Tag™ sequence [pET-40b(+)].

      DsbA and DsbC are periplasmic enzymes that catalyze the formation and isomerization of disulfide bonds, respectively (Rietsch 1996, Sone 1997, Missiakas 1994, Zapun 195, Raina 1997). In contrast to the pET-32 Trx•Tag™ series, which is designed to increase solubility in the cytoplasm, the DsbA•Tag and DsbC•Tag vectors enable potential periplasmic localization and thus may enhance solubility and proper folding in this non-reducing environment (Collins-Racie 1995).








      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      Catalogue Number70791
      Brand Family Novagen®
      References
      References

      Rietsch, A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13048. Sone, M., et al. 1997. J. Biol. Chem. 272, 10349. Missiakas, D., et al. 1994. EMBO J. 13, 2013. Zapun, A., et al. 1995. Biochemistry 34, 5075. Raina, S. and Missiakas, D. 1997. Ann. Rev. Microbiol. 51, 179. Collins-Racie, L.A., et al. 1995. Bio/Technology 13, 982.
      Product Information
      Components
      Fusion tagHis•Tag, S•Tag
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      70791 0

      Documentation

      pET Dsb Fusion System 40b Certificates of Analysis

      TitleLot Number
      70791

      References

      Reference overview

      Rietsch, A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13048. Sone, M., et al. 1997. J. Biol. Chem. 272, 10349. Missiakas, D., et al. 1994. EMBO J. 13, 2013. Zapun, A., et al. 1995. Biochemistry 34, 5075. Raina, S. and Missiakas, D. 1997. Ann. Rev. Microbiol. 51, 179. Collins-Racie, L.A., et al. 1995. Bio/Technology 13, 982.

      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB229 pET-40b Vector Map

      Related Products & Applications

      Related Products

      Catalog Number Description  
      70090 pET-39b(+) DNA - Novagen Show Pricing & Availability
      70091 pET-40b(+) DNA - Novagen Show Pricing & Availability
      70789 pET Dsb Fusion System 39b Show Pricing & Availability

      Related Products By: Application Facete

      Protein Expression

      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors