71125 Sigma-AldrichpET NusA Fusion System 44
Recommended Products
Overview
| Replacement Information |
|---|
| Description | |
|---|---|
| Overview | This product has been discontinued. pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression. Components: pET Expression Systems Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include: • 10 µg pET vector DNA (for each indicated plasmid) • 0.2 ml BL21 Glycerol Stock • 0.2 ml BL21(DE3) Glycerol Stock • 0.2 ml BL21(DE3)pLysS Glycerol Stock • 0.2 ml Induction Control Glycerol Stock Components: pET Expression Systems plus Competent Cells pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description: • 0.2 ml NovaBlue Competent Cells • 0.2 ml BL21(DE3) Competent Cells • 0.2 ml BL21(DE3)pLysS Competent Cells • 2 × 0.2 ml SOC Medium • 10 µl Test Plasmid These components are sufficient for up to 10 transformations in each host. Purification and Detection Reagents Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters. pET NusA Fusion System 44 The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag sequence for enhanced yields when purifying a target protein with His•Mag™ Agarose Beads from lysates generated with the BugBuster® HT Protein Extraction Reagent and PopCulture® Reagent (Novy 2002). N-terminal S•Tag and optional C-terminal HSV•Tag and His•Tag fusion sequences are also available for detection and purification of target proteins. The pET NusA Fusion System 44 is designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag™) protein. The solubility of many target proteins in the E. coli cytoplasm is increased when they are expressed NusA fusions. The pET-44a-c(+) vectors, which are derived from the corresponding pET-43.1 series, encode an additional N-terminal His•Tag® sequence for enhanced yields when purifying a target protein with His•Mag Agarose Beads from lysates generated with the BugBuster HT Reagent and PopCulture Reagent. N-terminal S•Tag™ and optional C-terminal HSV•Tag® and His•Tag fusion sequences are also available for versatile detection and purification of target proteins. Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product. |
| Catalogue Number | 71125 |
| Brand Family | Novagen® |
| References | |
|---|---|
| References | Harrison, R.G. 2000. inNovations 11, 4. Davis, G.D., et al. 1999. Biotechnol. Bioeng. 65, 382. Novy, R. and Drott, D. 2002. inNovations 14, 12. |
| Product Information | |
|---|---|
| Components | |
| Applications |
|---|
| Biological Information |
|---|
| Physicochemical Information |
|---|
| Dimensions |
|---|
| Materials Information |
|---|
| Toxicological Information |
|---|
| Safety Information according to GHS |
|---|
| Safety Information |
|---|
| Product Usage Statements |
|---|
| Storage and Shipping Information | |
|---|---|
| Ship Code | Shipped with Blue Ice or with Dry Ice |
| Toxicity | Standard Handling |
| Storage | ≤ -70°C |
| Do not freeze | Ok to freeze |
| Packaging Information |
|---|
| Transport Information |
|---|
| Supplemental Information |
|---|
| Specifications |
|---|
| Global Trade Item Number | |
|---|---|
| Catalog Number | GTIN |
| 71125 | 0 |
Documentation
pET NusA Fusion System 44 Certificates of Analysis
| Title | Lot Number |
|---|---|
| 71125 |
References
| Reference overview |
|---|
Harrison, R.G. 2000. inNovations 11, 4. Davis, G.D., et al. 1999. Biotechnol. Bioeng. 65, 382. Novy, R. and Drott, D. 2002. inNovations 14, 12. |
Citations
| Title | |
|---|---|
|
|
User Protocols
| Title |
|---|
| TB053 Academic and Non-profit Laboratory Assurance Letter |
| TB055 pET System Manual |
Vector Map
| Title |
|---|
| TB330VM pET-44a-c(+) Vector Map |