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69091 pIE1-4 DNA

69091
  
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      Overview

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      This product has been discontinued.

      We apologize for the inconvenience, but we do not currently have an alternative product.


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      The pIE1-4 vector is designed for constitutive expression of recombinant proteins from the baculovirus ie1 promoter in stably-transformed insect cells (1). The plasmid contains all promoter sequences known to be important for ie1-mediated gene expression in uninfected insect cells as well as the hr5 enhancer element. pIE1-4 lacks an ie1-driven translation initiation site; therefore, the ATG start codon must be supplied by the insert. To obtain stably-transformed cells, a pIE1-4 recombinant is cotransfected with pIE1-neo, which contains the Neo selectable marker under ie1 control.
      Catalogue Number69091
      Brand Family Novagen®
      References
      References1. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Prot. Exp. Pur. 8, 191–203.
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      Ship Code Blue Ice or Dry Ice
      Toxicity Standard Handling
      Storage FREEZER (-20°C)
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Global Trade Item Number
      Catalog Number GTIN
      69091 0

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      References

      Reference overview
      1. Jarvis, D.L., Weinkauf, C. and Guarino, L.A. (1996) Prot. Exp. Pur. 8, 191–203.

      Citations

      Title
    • Jones, G., et al. 2000. Deterin, a new inhibitor of apoptosis from Drosophila melanogaster. Journal of Biological Chemistry 275, 22157.