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71243 pIEx™-3 DNA

pIEx™-3 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: InsectDirect™ System- pIEx™-3 DNA

71243
  
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      Overview

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      Overview

      This product has been discontinued.





      The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-3 contains the coding sequence for the signal peptide of adipokinetic hormone (AKH) to allow secretion of the expressed protein. pIEx-3 also contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. Unique sites are shown on the circle map. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
      pIEx-3 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

      For sequencing or PCR of recombinants in pIEx-3, refer to the table below to choose the appropriate primers.
      Vector5' Primer3' Primeralt 5' Primer
      pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
      NA
      pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
      T7 Promoter Primer
      pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer
      Catalogue Number71243
      Brand Family Novagen®
      SynonymsInsectDirect™ System- pIEx™-3 DNA
      References
      Product Information
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      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
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      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      71243 0