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MABC1171-25UG Anti-ALK Antibody, clone ALK1

Anti-ALK, clone ALK1, Cat. No. MABC1171, is a mouse monoclonal antibody that detects ALK tyrosine kinase receptor and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-ALK Antibody, clone ALK1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ALK UniProt Number: Q9UM73
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MABC1171-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1171-25UG
      DescriptionAnti-ALK Antibody, clone ALK1
      Alternate Names
      • ALK tyrosine kinase receptor
      • EC: 2.7.10.1
      • Anaplastic lymphoma kinase
      • CD246
      Background InformationALK tyrosine kinase receptor (UniProt: Q9UM73; also known as EC: 2.7.10.1, Anaplastic lymphoma kinase, CD246) is encoded by the ALK gene (Gene ID: 238) in human. ALK tyrosine kinase receptor is a single-pass type I membrane protein that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the development and function of the nervous system. It is predominantly expressed in the central nervous system, intestine, and testis. It acts as a receptor for ligands pleiotrophin (PTN) and midkine (MDK). It is activated and homodimerizes by ligand-binding and subsequent phosphorylation. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. It is inactivated through dephosphorylation by receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) in the absence of any stimulation by a ligand. A chromosomal aberration involving ALK has been reported in non-Hodgkin lymphoma where the kinase is constitutively activated. Another chromosomal aberration involving ALK has also been linked with anaplastic large-cell lymphoma (ALCL). ALK tyrosine kinase receptor is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form. ALK tyrosine kinase receptor consists of an extracellular domain (aa 19-1038); a transmembrane domain (aa 1039-1059); and a cytoplasmic domain (aa 1060-1620).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-ALK, clone ALK1, Cat. No. MABC1171, is a mouse monoclonal antibody that detects ALK tyrosine kinase receptor and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected ALK in Western Blotting applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

      Immunohistochemistry Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

      Immunofluorescence Analysis: A representative lot detected ALK in Immunofluorescence applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated ALK in Immunoprecipitation applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).
      Biological Information
      ImmunogenHis-tagged full length human recombinant ALK tyrosine kinase receptor.
      CloneALK1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone ALK1 specifically detects human ALK tyrosine kinase receptor. It targets an epitope with in 102 amino acids from the C-terminal region.
      IsotypeIgG3κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ALK
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight176.44 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human anaplastic large cell lymphoma tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected ALK in human anaplastic large cell lymphoma tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1171-25UG 04054839540356