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ABE1298-100UL Anti-phospho BAF60C (Ser247)

Anti-phospho BAF60C (Ser247), Cat. No. ABE1298, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (BAF60C) phosphorylatd on Ser247 and has been tested for use in Peptide Inhibition Assay & WB.

Research Category: Epigenetics & Nuclear Function Gene Symbol: SMARCD3, BAF60C UniProt Number: Q6STE5
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ABE1298-100UL
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1298-100UL
      DescriptionAnti-phospho BAF60C (Ser247)
      Alternate Names
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3
      • 60 kDa BRG-1/Brm-associated factor subunit C
      • BRG1-associated factor 60C
      • BAF60C
      Background InformationSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (UniProt: Q6STE5; also known as 60 kDa BRG-1/Brm-associated factor subunit C, BRG1-associated factor 60C, BAF60C) is encoded by the SMARCD3 (also known as BAF60C) gene (Gene ID: 6604) in human. BAF60C plays a key role in ATP dependent nucleosome remodeling by SMARCA4 containing complexes. Two isoforms of BAF60C have been reported that are produced by alternative splicing. Both isoforms are expressed in brain, heart, kidney, placenta, prostate, salivary glands, spleen, testis, thyroid, trachea, and uterus. However, isoform 1 is expressed also in skeletal muscle and adipose tissue. BAF60C belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. BAF60C is also a component of nuclear Notch signaling and is required for the establishment of left-right asymmetry. Loss of BAF60C can lead to defects in the establishment of the left-right asymmetry cascade in mouse and zebrafish. It also serves a specific chromatin remodeling component for lipogenic gene transcription in liver. In response to insulin, it is phosphorylated at serine 247 by atypical PKC zeta//lambda, which causes translocation of BAF60C to the nucleus and allows a direct interaction of BAF60C with upstream stimulatory factor-1 (USF-1). (Ref.: Takeuchi, JK., et al. (2007). Proc. Natl. Acad. Sci. USA 104(3); 846-851; Wang, Y., et al. (2012). Mol. Cell 49(2); 283-297).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho BAF60C (Ser247), Cat. No. ABE1298, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3 (BAF60C) phosphorylatd on Ser247 and has been tested for use in Peptide Inhibition Assay & WB.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with NIH3T3 Nuclear Extract for peptide block analysis.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphorylated serine 247 of human BAF60C. The immunogen is conserved between both isoforms.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects murine BAF60C phosphorylated on Serine 247.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Bovine, Human, and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMARCD3
      • BAF60C
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~55 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 Nuclear Extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho BAF60C (Ser247) in NIH3T3 Nuclear Extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABE1298-100UL 04054839448157

      Documentation