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MABF3073-25UG Anti-SARS-CoV-2 Spike Protein Antibody, clone S3D8

Anti-SARS-CoV-2 Spike Protein, clone S3D8, Cat. No. MABF3073, is a mouse monoclonal antibody that detects spike glycoprotein in SARS-CoV-2 and is tested for use in ELISA, Immunofluorescence, Immunoprecipitation, and Neutralizing.

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MABF3073-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3073-25UG
      DescriptionAnti-SARS-CoV-2 Spike Protein Antibody, clone S3D8
      Alternate Names
      • Spike glycoprotein
      • S glycoprotein
      • E2
      • Peplomer protein
      Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Clone S3D8 exhibits a strong preference for tertiary structure of spike glycoprotein and is not suitable for Western blotting, but grants a remarkable performance in immunoprecipitation. It is shown to block the binding of Spike protein with ACE2 (IC50 = 225.6 ng/mL) and neutralize infection of VeroE6/TM2 cells by SARS-CoV-2 (IC50 = 139 ng/mL). (Ref.: Guo, Y., et al. (2021). J. Biol. Chem. 296; 100346).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-SARS-CoV-2 Spike Protein, clone S3D8, Cat. No. MABF3073, is a mouse monoclonal antibody that detects spike glycoprotein in SARS-CoV-2 and is tested for use in ELISA, Immunofluorescence, Immunoprecipitation, and Neutralizing.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Immunofluorescence
      • Neutralizing
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected SARS-CoV-2 Spike Protein in Immunofluorescence applications (Guo, Y., et al. (2021). J Biol Chem. 296:100346).

      Immunoprecipitation Analysis: A representative lot detected SARS-CoV-2 Spike Protein in Immunoprecipitation applications (Guo, Y., et al. (2021). J Biol Chem. 296:100346).

      ELISA Analysis: A representative lot detected SARS-CoV-2 Spike Protein in ELISA applications (Guo, Y., et al. (2021). J Biol Chem. 296:100346).

      Neutralizing: A representative lot neutralized SARS-CoV-2 Spike Protein in Neutralizing applications (Guo, Y., et al. (2021). J Biol Chem.;296:100346).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenSpike TM‐SBP protein (residue 1‐1208 aa) expressed in human Expi293 cells.
      EpitopeExtracellular domain
      CloneS3D8
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone S3D8 is a mouse monoclonal antibody that detects SARS-CoV-2 spike protein. It recognizes the intact tertiary structure of the spike protein.
      IsotypeIgG2a
      Species Reactivity
      • Virus
      Species Reactivity NoteSARS-CoV-2
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • S
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight141.18 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with recombinant SARS-CoV-2 spike protein.

      ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant Spike glycoprotein of SARS-CoV-2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF3073-25UG 04065267216591