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EZGRA-88K Human Ghrelin (active) ELISA

This Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.

Human Ghrelin (active) ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
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EZGRA-88K
96-well strip plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin (Active) HELISAFluorescent Colorimetric
      Description
      Catalogue NumberEZGRA-88K
      DescriptionHuman Ghrelin (active) ELISA
      Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      HS Code3002 15 90
      Precision, %
      • Inter-assay: Serum: 3.5–6.6% Plasma: 7.5–12.9%
      • Intra-assay: Serum: 1.6–3.6% Plasma: 0.9–7.5%
      Quality LevelMQ200
      Applications
      ApplicationThis Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Biological Information
      SpecificityHuman Ghrelin (active) 100% Des-Octanoyl Human Ghrelin not detectable Canine Ghrelin (active) 106% Porcine Ghrelin (active) 57% Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin (Active)
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • obestatin
      • MTLRP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • Serum: 84.4–88.4%
      • Plasma: 94.7–94.8%
      Physicochemical Information
      Sensitivity
      • 8 pg/mL (20 µL sample size)
      Linearity of DilutionSerum: 100.1–110.2% Plasma: 87.5–89.9%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      EZGRA-88K 08436037123016

      Documentation

      Protocols

      Title
      Protocol- EZGRA-88K|EZGRA-88BK
      Protocol: Human Ghrelin (Active)

      Human Ghrelin (active) ELISA SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms: Beyond fit for purpose
      Custom Immunoassay Menu of Services

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Range Sheet- AGR-117 and 217
      Quality Control Range Sheet- AGR-118 and 218
      Quality Control Ranges: Human Ghrelin (Active) ELISA - 96-Well Plate

      Data Sheet

      Title
      Full Service Custom-built Assays

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits