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69745 pET-23a(+) DNA - Novagen

69745
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69745-3
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) by the "plain" T7 promoter instead of the T7lac promoter and by the absence of the lacI gene. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB051). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69745
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      69745-3 04055977259063

      Documentation

      pET-23a(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69745

      Citations

      Title
    • Pinto, R., et al. 2007. Journal of Bacteriology 189, 6714.
    • Steinboeck, F., et al. 2007. Journal of Biochemistry (Tokyo) 141, 661.
    • Wu, T., et al. 2006. Procedings of the National Academy of Science 103, 11754.
    • Attinger, A., et al. 2005. Journal of Immunology 174, 3501.
    • Castanheira, P., et al. 2005. Journal of Biological Chemistry 280, 13047.
    • Hinckley, M.B., et al. 2005. Journal of Biological Chemistry 280, 30214.
    • Jang, S-I., et al. 2005. Journal of Cell Science 118, 781.
    • Prakash, P., et al. 2005. Journal of Biological Chemistry 280, 19641.
    • Sakthivel, S., et al. 2005. Journal of Biological Chemistry 280, 703.
    • Olga Vitavska, Hans Merzendorfer and Helmut Wieczorek. 2005. Journal of Biological Chemistry 280, 1070.
    • Naohide Watanabe and Eric Lam. 2005. Journal of Biological Chemistry 280, 14691.
    • Bonney Wilkinson, Ruoyu Xiao and Hiram F. Gilbert. 2005 Journal of Biological Chemistry 280, 11483.
    • Xiong, L., et al. 2005. Journal of Biological Chemistry 280, 7070.
    • Yagi, H., et al. 2005. Journal of Biological Chemistry 280, 38609.
    • Gallagher, C.E., et al. 2004. Plant Physiology 135, 1776.
    • Iwona K. Wower, Christian Zwieb and Jacek Wower. 2004. Journal of Biological Chemistry 279, 54202.
    • Assen Marintchev, Michael R. Gryk and Gregory P. Mullen. 2003. Nucleic Acids Research 31, 580.
    • Maeda, Y., et al. 2002. Infection and Immunity 70, 4106.
    • Zimmerman, K.K., et al. 1998. Protein Expression and Purification 14, 395-.
    • Black, S.M., et al. 1997. Journal of Clinical Investigation 100, 1448.
    • Shanley, T.P., et al. 1997. Journal of Immunology 158, 3439.
    • User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB051VM pET-23a-d(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors