71177 Sigma-AldrichRosetta-gami™ B Competent Cell Set - Novagen
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Overview
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Pricing & Availability
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| 71177-3 |
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Glass bottle | 1 set |
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| Description | |
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| Overview | Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. |
| Genotypes: Rosetta-gami B F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC gor522::Tn10 trxB pRARE (CamR, KanR, TetR) Rosetta-gami B(DE3) F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR) Rosetta-gami B(DE3)pLysS F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pLysSRARE (CamR, KanR, TetR) This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com. |
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| Catalogue Number | 71177 |
| Brand Family | Novagen® |
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| Product Information | ||
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| • | 2 × 0.2 ml | Rosetta-gami B Competent Cells |
| • | 2 × 0.2 ml | Rosetta-gami B(DE3) Competent Cells |
| • | 2 × 0.2 ml | Rosetta-gami B(DE3)pLysS Competent Cells |
| • | 4 × 2 ml | SOC Medium |
| • | 2 ng | Test Plasmid |
| Quality Level | MQ100 | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | ≤ -70°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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| Catalog Number | GTIN |
| 71177-3 | 04055977254013 |
Documentation
Rosetta-gami™ B Competent Cell Set - Novagen SDS
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Rosetta-gami™ B Competent Cell Set - Novagen Certificates of Analysis
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Brochure
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| Express yourself |
User Protocols
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| TB009 Competent Cells |
| TB053 Academic and Non-profit Laboratory Assurance Letter |