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MABE351 Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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MABE351
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICC, ChIP-seq, ChIPMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABE351
      DescriptionAnti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, acetylation of H3K4 is regulated by Gcn5 and the Mst2 complex, and is crucial to the activation of DNA damage checkpoint.
      References
      Product Information
      FormatAscites
      Control
      • HeLa acid extract
      PresentationMouse monoclonal IgG1 in ascites containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation Analysis: A representative lot was used to immunoprecipitate acetyl-Histone H3 (Lys4) in IP.

      Immunocytochemistry Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ICC.

      Chromatin Immunoprecipitation Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ChIP.

      Western Blot Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in WB.
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys14.
      Cloneclone 13HH3-1A5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human.
      Demonstrated to react in Mouse ESCs in ChIP-seq in Karmodiya et al. BMC Genomics 2012, 13:424.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed at ~23 kDa and ~35 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H3 (Lys4) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE351 04053252697760

      Documentation

      Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys14), -2520566 2520566
      Anti-acetyl-Histone H3 (Lys14), -2665808 2665808
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 3013345
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 2298448 2298448
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 3508428 3508428
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 3853802 3853802
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 4204627 4204627
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - Q2029943 Q2029943
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 -2705156 2705156
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 Monoclonal Antibody 2959567
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      References

      Reference overviewApplicationSpeciesPub Med ID
      H3K9 and H3K14 acetylation co-occur at many gene regulatory elements, while H3K14ac marks a subset of inactive inducible promoters in mouse embryonic stem cells.
      Karmodiya, Krishanpal, et al.
      BMC Genomics, 13: 424 (2012)  2012

      Show Abstract       		Chromatin Immunoprecipitation (ChIP)Mouse22920947 22920947

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies