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06-942-AF647 Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 647 Conjugate

Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate Antibody, Cat. No. 06-942-AF647, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: Q16695
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06-942-AF647
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, FCRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue Number06-942-AF647
      DescriptionAnti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Lys9-acetylated
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit polyclonal antibody in PBS with 1.5% BSA with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate Antibody, Cat. No. 06-942-AF647, is a highly specific rabbit polyclonal antibody that targets histone H3 Lys9 acetylation (H3K9ac) and has been tested in Flow Cytometry and Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 2 µL from a representative lot detected cell population with histone H3 Lys9 acetylation (H3K9ac) among one million HEK293 cells.

      The unconjugated antibody (Cat. No. 06-492) is shown to be suitable also for Western blotting, dot blot, flow cytometry, chromatin immunoprecipitation (ChIP) and ChIP-seq applications.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityHistone H3 acetylated at position 9.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H3A
      • H3FA
      • HIST1H3B
      • H3FL
      • HIST1H3C
      • H3FC
      • HIST1H3D
      • H3FB
      • HIST1H3E
      • H3FD
      • HIST1H3F
      • H3FI
      • HIST1H3G
      • H3FH
      • HIST1H3H
      • H3FK
      • HIST1H3I
      • H3FF
      • HIST1H3J
      • H3FJ
      Modifications
      • Acetylation
      Purification MethodProtein A purified.
      UniProt Number
      Molecular Weight~17 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected histone H3 Lys9 acetylation (H3K9ac) in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      06-942-AF647 04054839056215

      Documentation

      Anti-acetyl-Histone H3 (Lys9) Antibody, Alexa Fluor® 647 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys9), -Q2736873 Q2736873
      Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate - 3172392 3172392
      Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate - 3275490 3275490
      Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate - 3328181 3328181
      Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate - 3907018 3907018
      Anti-acetyl-Histone H3 (Lys9), Alexa Fluor® 647 Conjugate - 4173638 4173638