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07-350-25UG Anti-AMPK a 1

Anti-AMPK alpha 1, Cat. No. 07-350 is rabbit polyclonal antibody that detects 5'-AMP-activated protein kinase catalytic subunit alpha-1 and is tested for use in Western Blotting and Immunohistochemistry.

Anti-AMPK a 1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: PRKAA1, MGC57364, AMPK, MGC33776, AMPKa1, AMPK1 UniProt Number: Q13131
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07-350-25UG
25 μg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-350-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-AMPK a 1
      Alternate Names
      • 5'-AMP-activated protein kinase, catalytic alpha-1 chain
      • AMP -activate kinase alpha 1 subunit
      • AMP-activated protein kinase, catalytic, alpha-1
      • AMPK alpha 1
      • AMPK alpha-1 chain, protein kinase
      • AMP-activated, alpha 1 catalytic subunit
      Background Information5'-AMP-activated protein kinase catalytic subunit alpha-1 (UniProt: Q13131; also known as EC:2.7.11.1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1) is encoded by the PRKAA1 (also known as AMPK1) gene (Gene ID: 5562) in human.AMPK is a heterotrimer composed of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). It also contains an autoinhibitory sequence that shows some sequence similarity with the ubiquitin-associated domains and it represses kinase activity. AMPK is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes, including inhibition of protein, carbohydrate and lipid biosynthesis, and reduces cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK is shown to regulate insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3 and it stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter GLUT4 to the plasma membrane. In the liver it acts as a key regulator of glucose homeostasis by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. AMPK is activated by phosphorylation on Thr183. Binding of AMP to non-catalytic gamma subunit results in allosteric activation, inducing phosphorylation on Thr183. AMP-binding to gamma subunit also sustains activity by preventing dephosphorylation of Thr183.
      References
      Product Information
      FormatPurified
      Control
      • A431cell lysate, or lysate of HeLa cells treated with heat shock
        Included Positive Antigen Control: Catalog # 12-301, Non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationProtein A purified rabbit in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
      Quality LevelMQ100
      Applications
      ApplicationAnti-AMPK alpha 1, Cat. No. 07-350 is rabbit polyclonal antibody that detects 5'-AMP-activated protein kinase catalytic subunit alpha-1 and is tested for use in Western Blotting and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: 2 ug/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.

      Immunohistochemistry (Paraffin) Analysis: 1:250 dilution of this antibody detected AMPK α1 in human gallbladder tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human AMPK catalytic subunit alpha-1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects AMPK1 alpha subunit in human. It targets an epitope within 17 amino acids from the C-terminal half of human AMPK1.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
      Gene Symbol
      • PRKAA1
      • MGC57364
      • AMPK
      • MGC33776
      • AMPKa1
      • AMPK1
      Purification MethodProtein A chromatography
      UniProt Number
      Molecular Weight~63 kDa observed; 64.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      07-350-25UG 04054839337451