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MABN2557-25UL Anti-Neurofibromin NF-1 Antibody, clone NF1-A 376G3

Anti-Neurofibromin NF-1, clone NF1-A 376G3, Cat. No. MABN2557, is a mouse monoclonal antibody that detects Neurofibromin and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MABN2557-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2557-25UL
      DescriptionAnti-Neurofibromin NF-1 Antibody, clone NF1-A 376G3
      Alternate Names
      • Neurofibromatosis-related protein NF-1
      Background InformationNeurofibromin (UniProt: P21359; also known as Neurofibromatosis-related protein NF-1) is encoded by the NF1 gene (Gene ID: 4763) in human and widely expressed in brain, peripheral nerves, lung, colon, breast, and muscle tissues. Germline NF1 loss-of-function mutations cause Neurofibromatosis type I. Somatic NF1 loss is also frequently detected in a wide range of tumors. Neurofibromin is a GTPase-activating protein (GAP) that negatively regulates RAS/MAK pathway by enhancing the hydrolysis of Ras-bound GTP. Its GAP domain is localized to amino acids 1235-1451. It also has a CRAL-TRIO domain (aa 1580-1738) that facilitates its binding to phospholipids. Neurofibromin has also been shown to serve as an estrogen receptor alpha (ER-a) transcriptional co-repressor through leucine/isoleucine-rich motifs, and this activity is independent of the GAP activity. Its depletion can thus cause estradiol hypersensitivity and tamoxifen agonism, which correlate with poor outcomes seen in ER+ breast cancer patients. Mutations in NF1 gene are known to cause Neurofibromatosis type I that involved tumors in the peripheral nervous system and fibromatous skin tumors. Some mutations are also known to cause colorectal cancer and juvenile leukemia. Six different isoforms of Neurofibromin have been described that are produced by alternative splicing. (Ref.: Rossi, S., et al. (2018). Mod. Pathol. 31(1), 160-168).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Neurofibromin NF-1, clone NF1-A 376G3, Cat. No. MABN2557, is a mouse monoclonal antibody that detects Neurofibromin and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Neurofibromin NF-1 in Immunoprecipitation applications (Zheng, Z.Y., et. al. (2020). Cancer_Cell. 37(3):387-402).

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Neurofibromin NF-1 in MCF-7 cell lysate.

      Immunocytochemistry Analysis: A representative lot detected Neurofibromin NF-1 in Immunocytochemistry applications (Zheng, Z.Y., et. al. (2020). Cancer_Cell. 37(3):387-402).

      Western Blotting Analysis: A representative lot detected Neurofibromin NF-1 in Western Blotting applications (Zheng, Z.Y., et. al. (2020). Cancer_Cell. 37(3):387-402).

      Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Neurofibromin NF-1 in MCF-7 and T47D cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 369 amino acids from the C-terminal region of human.
      EpitopeC-terminus
      CloneNF1-A 376G3
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone NF1-A 376G3 is a mouse monoclonal antibody that detects Neurofibromin. It targets an epitope within the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NF1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~320 kDa observed; 319.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC-12 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Neurofibromin NF1 in PC-12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2557-25UL 04061842414581

      Documentation

      Anti-Neurofibromin NF-1 Antibody, clone NF1-A 376G3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofibromin NF-1 Antibody, clone NF1-A 376G3 Certificates of Analysis

      TitleLot Number
      Anti-Neurofibromin NF-1, clone NF1-A 376G3 - Q3490942 Q3490942

      References

      Reference overviewPub Med ID
      Proteogenomic Approaches for the Identification of NF1/Neurofibromin-depleted Estrogen Receptor-positive Breast Cancers for Targeted Treatment.
      Kim, B-J., et al.
      Cancer research communications  3  1366-1377  2023

      Full Text Article      		PMC10370361 PMC10370361
      Neurofibromin Is an Estrogen Receptor-α Transcriptional Co-repressor in Breast Cancer
      Ze-Yi Zheng 1 , Meenakshi Anurag 1 , Jonathan T Lei 2 , Jin Cao 3 , Purba Singh 1 , Jianheng Peng 4 , Hilda Kennedy 1 , Nhu-Chau Nguyen 1 , Yue Chen 5 , Philip Lavere 3 , Jing Li 1 , Xin-Hui Du 6 , Burcu Cakar 1 , Wei Song 1 , Beom-Jun Kim 1 , Jiejun Shi 1 , Sinem Seker 1 , Doug W Chan 7 , Guo-Qiang Zhao 8 , Xi Chen 3 , Kimberly C Banks 9 , Richard B Lanman 9 , Maryam Nemati Shafaee 1 , Xiang H-F Zhang 7 , Suhas Vasaikar 1 , Bing Zhang 1 , Susan G Hilsenbeck 1 , Wei Li 1 , Charles E Foulds 10 , Matthew J Ellis 11 , Eric C Chang
      Cancer Cell  37(3)  387-402  2020

      Show Abstract       		32142667 32142667