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MABN2719-25UG Anti-Neurofilament M/NEFM Antibody, clone 2H3

Anti-Neurofilament M/NEFM, clone 2H3, Cat. No. MABN2719, is a mouse monoclonal antibody that detects Neurofilament M and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABN2719-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2719-25UG
      DescriptionAnti-Neurofilament M/NEFM Antibody, clone 2H3
      Alternate Names
      • Neurofilament medium polypeptide
      • NF-M
      • 160 kDa neurofilament protein
      • Neurofilament 3
      • Neurofilament triplet M protein
      Background InformationNeurofilament medium polypeptide (UniProt: P12839; also known as NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein) is encoded by the Nefm (also known as Nef3, Nfm) gene (Gene ID: 24588) in rat. Neurofilaments are intermediate filaments that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. They are built from three intertwined protofibrils, which themselves are composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neurofilaments contain a number of repeats of the tripeptide KSP (Lys-Ser-Pro) and NF-M is phosphorylated on a number of the serine residues in this motif by protein kinase A and C. Phosphorylation of NF-M results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Deletion of the phosphorylated tail domain of NF-M is reported to inhibit radial growth of axons and reduce their conduction velocities. However, mice expressing NF-M subunits that lack phosphorylation at KSP sites along the tail domain have normal axon calibers and conduction velocities, indicating that the NF-M tail domain, but not tail phosphorylation is crucial for axon radial growth. The level of phosphorylation is shown to be altered developmentally and coincides with a change in the neurofilament function. Mutations in the rod domain region of NF-M have been identified in early onset Parkinson s disease. Also, in the brains of Alzheimer s disease patients, a reciprocal relationship between O-GlcNAcylation and phosphorylation of NF-M has been reported where reduced O-GlcNAcylation is accompanied with increased KSP phosphorylation in NF-M. (Ref.: Yuan, A., et al. (2017). Cold Spring Harb. Perspect. Biol. 9(4); a018309; Yuan, A., et al. (2012). J. Cell Sci. 125(14); 3257-3263).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Neurofilament M/NEFM, clone 2H3, Cat. No. MABN2719, is a mouse monoclonal antibody that detects Neurofilament M and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected Neurofilament medium polypeptide (NF-M) in mouse brain tissue extract.

      Immunohistochemistry Applications: A representative lot detected Neurofilament medium polypeptide (NF-M) in Immunohistochemistry applications (Clugston, R.D., et al. (2010). Am J Respir Cell Mol Biol. 42(3):276-85; Lysakowski, A., et al. (2011). J Neurosci. 31(27):10101-14; Kridsada, K., et al. (2018). Cell Rep. 23(10):2928-2941; Latremoliere, A., et al. (2018). Cell Rep. 24(7):1865-1879.e.9).

      Immunocytochemistry Analysis: A representative lot detected Neurofilament medium polypeptide (NF-M) in Immunocytochemistry applications (Latremoliere, A., et al. (2018). Cell Rep. 24(7):1865-1879.e.9).

      Immunofluorescence Analysis: A representative lot detected Neurofilament medium polypeptide (NF-M) in Immunofluorescence applications (Latremoliere, A., et al. (2018). Cell Rep. 24(7):1865-1879.e.9).

      Western Blotting Analysis: A representative lot detected Neurofilament medium polypeptide (NF-M) in Western Blotting applications (Fernandez-Cerado, C., et al. (2021). J Neural Transm (Vienna). 128(4):575-587).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenMembrane preparations from E14-E15 rat brain tissue.
      EpitopeUnknown
      Clone2H3
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2H3 is a mouse monoclonal antibody that detects Neurofilament-M (NEFM)
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nefm
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~160 kDa observed; 95.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain tissue extracts.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Neurofilament medium polypeptide (NF-M) in Rat brain tissue extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN2719-25UG 04065270453594