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MABT521 Anti-SMAD3, clone 5G11 Antibody

Anti-SMAD3, clone 5G11 Antibody is an antibody against SMAD3 for use in Western Blotting and Immunocytochemistry.

Anti-SMAD3, clone 5G11 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: SMAD3, MADH3 UniProt Number: P84022
MABT521
100 µL  
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      Overview

      Replacement Information

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABT521
      Replaces04-1035
      DescriptionAnti-SMAD3, clone 5G11 Antibody
      Alternate Names
      • Mothers against decapentaplegic homolog 3
      • MAD homolog 3
      • Mad3
      • Mothers against DPP homolog 3
      • hMAD-3
      • JV15-2
      • SMAD family member 3
      • SMAD 3
      • Smad3
      • hSMAD3
      • SMAD3
      Background InformationMembers of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include Smad1, 2, 3, 5 and 8, the common-mediator SMAD (co-SMAD), SMAD4, and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, SMAD2 and SMAD3 become phosphorylated at their carboxyl termini (Ser465 and 467 on SMAD2; Ser423 and 425 on SMAD3) by TGF-β Receptor I. Phosphorylated SMAD 2/3 can complex with SMAD4, translocate to the nucleus and regulate gene expression.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SMAD3, clone 5G11 Antibody is an antibody against SMAD3 for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected SMAD3 in NIH/3T3 cells.
      Biological Information
      ImmunogenPurified recombinant fragment of human SMAD3 expressed in E. Coli.
      Clone5G11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SMAD3
      • MADH3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected SMAD3 in A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT521 04055977171518

      Documentation

      Anti-SMAD3, clone 5G11 Antibody Certificates of Analysis

      TitleLot Number
      Anti-SMAD3, clone 5G11 VP1606072A
      Anti-SMAD3, clone 5G11 - VP1911085 VP1911085
      Anti-SMAD3, clone 5G11 - VP2304201 VP2304201
      Anti-SMAD3, clone 5G11 -VP1512155 VP1512155
      Anti-SMAD3, clone 5G11 -VP1606072 VP1606072
      Anti-SMAD3, clone 5G11 Monoclonal Antibody VP1806294

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

      Related Products & Applications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies