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MAB3574-C Anti-Vinculin Antibody, clone VIIF9 (7F9), Ascites Free

Anti-Vinculin Antibody, clone VIIF9 (7F9), Ascites Free
is an antibody against Vinculin for use in Western Blotting
, Immunohistochemistry (Paraffin, Immunofluorescence

Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: VCL, MVCL, CMD1W, CMH15 UniProt Number: P18206
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MAB3574-C
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, RbWB, IH(P), IF, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3574-C
      DescriptionAnti-Vinculin Antibody, clone VIIF9 (7F9), Ascites Free
      Alternate Names
      • Metavinculin
      • Meta-vinculin
      • MV
      • Vinculin
      Background InformationMetavinculin (MV) and its a.a. 916-983 truncated splice variant Vinculin (UniProt P18206) are cytoskeleton proteins encoded by the VCL (also known as MVCL, CMD1W, CMH15) gene (Gene ID 7414) in human. The N-terminal globular head domain (a.a. 2-835) of Vinculin/VM contains binding sites for talin and α-actinin as well as a tyrosine phosphorylation site, while their C-terminal Tail region (a.a. 879-1134 of VM; a.a. 879-1066 of Vinculin) contains binding sites for F-actin, paxillin, and lipids. Vinculin is associated with focal adhesion and adherens junctions, which are complexes that nucleate actin filaments and crosslinkers between the external medium, plasma membrane, and actin cytoskeleton. The loss of vinculin disrupts focal adhesion complex formation and prevents cell adhesion and spreading, accompanied by reduced stress fiber formation and inhibition of lamellipodia extension. Smooth muscles and skeletal muscles in their well-differentiated, contractile state co-express vinculin and meta-vinculin. Both vinculin isoforms co-localize in muscular adhesive structures, such as dense plaques in smooth muscles, intercalated discs in cardiomyocytes, and costameres in skeletal muscles.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Vinculin Antibody, clone VIIF9 (7F9), Ascites Free
      is an antibody against Vinculin for use in Western Blotting
      , Immunohistochemistry (Paraffin, Immunofluorescence

      Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Viculin in 10 µg of A431 cell lysate.
      Immunohistochemistry Analysis: An 1:1,000 dilution from a representative lot detected Viculin in human kidney and colon tissue sections.
      Western Blotting Analysis: A representative lot detected vinculin in HEK293 and HepG2 lysates (Veronese, A., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(12):4840-4845).
      Western Blotting Analysis: Clone VII F7 reacted equally with human vinculin and meta-vinculin by immunoblotting and recognized all human vinculin (α, α', β, γ) and meta-vinculin (α & β) variants resolved by isoelectrofocusing (Glukhova, M.A., et al. (1990). J. Biol. Chem. 265:13042-13046).
      Immunofluorescence Analysis: A representative lot immunostained the focal adhesion protein vinculin in acetone-fixed frozen mouse skeletal and cardiac muscle sections by fluorescent immunohistochemistry (Belkin, A.M., et al. (1996). J. Cell Biol. 132(1-2):211-226).
      Immunocytochemistry Analysis: A representative lot detected an increased average size and number of focal adhesions per cell following miR-200-mediated genes knockdown by fluorescent immunocytochemistry staining of paraformaldehyde-fixed, Triton X-100-permeabilized MDA-MB-231 human breast cancer cells (Bracken, C.P., et al. (2014). EMBO J. 33(18):2040-2056).
      Immunocytochemistry Analysis: A representative lot immunostained the focal adhesion protein vinculin in paraformaldehyde-fixed, Triton X-100-permeabilized human fibroblasts by fluorescent immunocytochemistry (Addad, S., et al. (2011). Mar. Drugs. 9(6):967-983).
      Immunocytochemistry Analysis: A representative lot immunostained the focal adhesion protein vinculin in fixed human intestinal epithelial cells (HIECs) by fluorescent immunocytochemistry (Gagné , D., et al. (2010). J. Cell Physiol. 222(2):387-400).
      Immunocytochemistry Analysis: Representative lots immunostained the focal adhesion protein vinculin in paraformaldehyde-fixed, Triton X-100-permeabilized REF52 rat embryonic fibroblasts, mouse skin fibroblasts, rabbit chondrocytes, and human MCF-7 cells by fluorescent immunocytochemistry (Akimov, S.S., et al. (2000). J. Cell Biol. 148(4):825-838; Jurjus, R.A., et al. (2008). Wound Repair Regen. 16(5):649-660; Yamamoto, K., et al. (2007). Biomaterials. 28(10):1838-1846; Poppe, M., et al. (2007). Oncogene. 26(24):3462-3472).
      Biological Information
      ImmunogenHuman uterus meta-vinculin.
      CloneVIIF9 (7F9)
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone VII F9 (7F9) reacted equally with human vinculin and meta-vinculin by immunoblotting and recognized all vinculin (α, α', β, γ) and meta-vinculin (α & β) variants resolved by isoelectrofocusing (Glukhova, M.A., et al. (1990). J. Biol. Chem. 265:13042-13046).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VCL
      • MVCL
      • CMD1W
      • CMH15
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~130 kDa observed. Target band size appears larger than the calculated molecular weights of 116.7 kDa (Vinculin; isoform 1) and 123.80 kDa (Metainculin; isoform 2).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Viculin in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB3574-C 04055977331271

      Documentation

      Anti-Vinculin Antibody, clone VIIF9 (7F9), Ascites Free
      SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vinculin Antibody, clone VIIF9 (7F9), Ascites Free
      Certificates of Analysis

      TitleLot Number
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free 3167568
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free 2903962
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 3840849 3840849
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 3856165 3856165
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 3897600 3897600
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 3959462 3959462
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 4008136 4008136
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 4015613 4015613
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free - 4092803 4092803
      Anti-Vinculin, clone VIIF9 (7F9), Ascites Free -Q2653194 Q2653194
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies