Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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You can now customize another kit, choose a premixed kit, check out or close the ordering tool.
For gentle, efficient, non-mechanical extraction of soluble proteins from bacterial cells, use BugBuster® Protein Extraction Reagents. This proprietary, innovative, Tris-buffer based mixture of non-ionic and zwitterionic detergents and other ingredients is capable of perforating cell walls without denaturing protein. It provides a simple, rapid, low-cost alternative to mechanical methods, such as French Press or sonication, for releasing expressed target protein for purification or other applications. Simply add the BugBuster® Reagent to your cell pellet and incubate at room temperature for 10 minutes.
Features & Benefits
No special sample preparation or instruments are needed
Standard reagent is supplied as a Tris-buffered "1X" ready-to-use liquid
Stable at room temperature
Performance can be enhanced using Benzonase® Nuclease and rLysozyme™ Solution
Fully compatible with GST•Bind™, Ni-NTA His•Bind®, S•Tag™, Strep•Tactin, and T7•Tag® Resins, or several other chromatography matrices for affinity purification
Applications
Protein Extraction, Protein Sample Preparation, Bacterial Cell Lysis